Fig. 7
The effect of 60 % ASMDM and HLCC monolayers on B. cenocepacia growth. Confluent HLCCs or wells without HLCCs were exposed to 60 % ASMDM diluted in Ham’s F-12 complete medium, infected with B. cenocepacia at MOI 5 or mock-infected with saline, and incubated at 37 °C (7 % CO2). After 24 h, the bacterial density (CFU/mL) of each well was compared to the starting bacterial density (T0). There are statistically significant differences in mean CFU/mL ratio between the different treatments (ANOVA, p = 0.0003). A Tukey-HSD post-hoc test showed the mean CFU/mL ratio in infected 100 % ASMDM is significantly different than mock-infected HLCCs + medium, mock-infected HLCCs + 60 % ASMDM, infected or mock-infected medium, and mock-infected 100 % ASMDM (p = 0.0024 for all). The mean CFU/mL ratio in infected HLCCs + 60 % ASMDM is significantly different than mock-infected HLCCs + medium, mock-infected HLCCs + 60 % ASMDM, mock-infected medium, and mock-infected 100 % ASMDM (p = 0.0354). Values are averages of three independent experiments with duplicate wells. Bars show standard deviation. Variables with different letters are statistically different (oneway ANOVA, p < 0.05)