Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda

Table 1 Primers used to amplify the fragments of the genes encoding the toxins and adhesins

Target	Primer sequence (5’ to 3’)	Conc. (pMol/μL)	Product size (bp)	Reference
STa	F: TTT CCC CTC TTT TAG TCA GTC AAC TG	0.05	160	[55]
STa	R: GGC AGG ATT ACA ACA AAG TTC ACA G	0.05
STb	F: GCC TAT GCA TCT ACA CAA TCA	0.05	114	[55]
STb	R: TGC TCC AGC AGT ACC ATC TCT AAC CC	0.05
LT	F: CCG GAT TGT CTT CTT GTA TGA	0.3	236	[56]
LT	R: TGT TCC TCT CGC GTG AT	0.3
F6	F: TCT GCT CTT AAA GCT ACT GG	0.1	333	[57]
F6	R: AAC TCC ACC GTT TGT ATC AG	0.1
F4	F: ATC GGT GGT AGT ATC ACT GC	0.5	601	[57]
F4	R: AAC CTG CGA CGT CAA CAA GA	0.5
EAST1	F: TGC CAT CAA CAC AGT ATA TC	0.2	107	[56]
EAST1	R: GAG TGA CGG CTT TGT AGT C	0.2
Stx2e	F: CCA GAA TGT CAG ATA ACT GGC GAC	0.1	322	[55]
Stx2e	R: GCT GAG CAC TTT GTA ACA ATG GCT G	0.1
F41	F: GCA TCA GCG GCA GTA TCT	0.2	380	[58]
F41	R: GTC CCT AGC TCA GTA TTA TCA CCT	0.2
F5	F: TGG GAC TAC CAA TGC TTC TG	0.2	450	[57]
F5	R: TAT CCA CCA TTA GAC GGA GC	0.2
F18	F: GTG AAA AGA CTA GTG TTT ATT TC	0.7	510	[59]
F18	R: CTT GTA AGT AAC CGC GTA AGC	0.7
AIDA-I	F: TGCAAACATTAAGGGCTCG	0.05	450	[19]
AIDA-I	R: CCGGAAACATTGACCATACC	0.05

ISSN: 1471-2180