Fig. 2

Evaluation of expression conditions. rCtE-MOMP with a PelB leader sequence constructed in the pAVE029 vector was evaluated. a Surface expression (FACS GFI) of rCtE-MOMP after different IPTG induction times (T = 0 and T = 4 h) when expressed at different temperatures (37 °C = circles, 30 °C = squares, 25 °C = triangles, 16 °C = downward pointing triangles). b Cell concentration (OD₅₉₀) at different temperatures (37 °C = circles, 30 °C = squares) after different IPTG induction times (T = 0 and T = 4 h). Geometric means of three independent experiments were plotted with error bars representing standard deviation. c Cell concentration (OD₅₉₀) and surface expression (FACS GFI) at harvest with different cell densities (OD₅₉₀) at IPTG induction (T = 0). d Surface expression (FACS GFI) with different cell culture media. Geometric means of replicates were plotted with error bars representing standard deviation