Strains or plasmids | Description | Source |
---|---|---|
Plasmids | ||
 pQK663 | Asd+ vector, p15A ori, specr | Derived from pYA3332 [26] |
 pQK163 | Insertion of crp into pQK663 | This work |
 pET-32a-crp | For the expression of P. multocida Crp | This work |
 pMC-Express | A broad host-range shuttle vector derived from pMIDG100, chloramphenicolr | [27] |
 pYA4278 | pRE112 derivative, sacB mobRP4 R6K ori Cm+ | [29] |
 pQK174 | pYA4278-Δcrp | This work |
 pQK175 | pYA4278-Δcrp::kan, for deletion of crp in P. multocida 0818 | This work |
 pQK176 | Insertion of crp into pMC-Express | This work |
Strains | ||
 S184 | S. Typhimurium Δasd66 | Lab collection |
 S411 | S. Typhimurium Δasd66 Δcrp89 | Lab collection |
  P. multocida 0818 | Wild-type and virulent. Capsular serotype A:1. | Lab collection |
 S416 | P. multocida 0818 Δcrp::kanR | This work |
 χ7232 | E.coli K-12, endA1 hsdR17 (rK-, mK +) glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR (ϕ80dlac Δ(lacZ)M15) | [30] |
 χ7213 | E.coli K-12, thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ∆asdA4 ∆zhf-2::Tn10 | [30] |