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Table 3 Strains and plasmids used in this work

From: Transcriptomic analysis of a classical model of carbon catabolite regulation in Streptomyces coelicolor

Strains/plasmids

Characteristics

Reference

  S. coelicolor M145

SCP1− SCP2−

[61]

  S. coelicolor ∆glk

SCP1− SCP2− glk::aacc4

This work

  S. coelicolor Zm

S. coelicolor ∆glk, harboring the plasmid pUNAMZm

This work

  S. coelicolor 702

S. coelicolor ∆glk, harboring the plasmid pIJ702

This work

  E. coli ET12567

F-dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44

[62]

  E. coli DH5α

Invitrogen

Plasmids

 pIJ702

Derived from pIJ101 with an estimated copy number of approximately 50

[16]; John Innes Centre

 pIJ2442

Derivative of pUC19 containing the1.7 pb PstI-XbaI Z. mobilis glk fragment

[16]; John Innes Centre

 pUNAM Zm

Plasmid pIJ702 harboring the 1.7 pb PstI-XbaI Z. mobilis glk fragment from pIJ2442

This work