Table 2 List of primers used to study lipoprotein genes expression in Spiroplasma citri GII-3
From: Differential expression of Spiroplasma citri surface protein genes in the plant and insect hosts
Name | Primers sequences (5′ → 3′) | Gene product | Amplicon length (bp) | Annealing temperature (°C) | Efficiency (%) |
|---|---|---|---|---|---|
pSci4_02 | GGCAATGACTTCAAGTTCGTG and TGTTTTCTCTTACTGTTGATGG | Hypothetical lipoprotein | 221 | 52 | 99.9 |
pSci4_06 | ATCAGTTAACAATGCTTCTGAG and TATCAGGCCTATCTTTACTATC | Hypothetical lipoprotein | 334 | 52 | 91.0 |
pSci6_18 | AGTGTTTCGCTCGGTTCTAG and GCATTTGCTTCACCAGATTTC | Truncated adhesion-related protein | 173 | 60 | 93.9 |
SPICI01A_047 | GATGTACGAATTCGCCAA and TCGATTCGTTGTTTTGCTTC | Hypothetical lipoprotein | 563 | 52 | 102.8 |
SPICI02_046 | TGCAACAACCAAGTTTCCAAG and TAGCAAGAACCGTATTTCCATG | Hypothetical lipoprotein | 288 | 60 | 95.9 |
SPICI03_030 | AGTAACATCACCAACCTTATTG and ATCGGTTGCTATTGTACCATC | Hypothetical lipoprotein | 219 | 60 | 103.0 |
SPICI03_098 | GTTTACAGGGAGGGCGAATG and TTGCAAGATAACGTGCTGATTG | Hypothetical lipoprotein | 573 | 60 | 100.2 |
SPICI03_180 | TTGGGAAAAGGCAGTTGGTAG and CTGTTCGCCCAATATTAGGTC | Hypothetical lipoprotein | 659 | 60 | 100.0 |
SPICI03_317 | GAAATAGTTTTGATAATGAGTTTAG and GCAGTGTTAAACATTACAAAATC | Hypothetical lipoprotein | 184 | 52 | 108.4 |
SPICI04_017 | CACCAGTTTCAAACCCAAC and AATTACTGCTGATTCATTAGG | Hypothetical lipoprotein | 86 | 60 | 99.7 |
SPICI04_108 | ACTTCGGCTTCTATTACTTCAG and CCTGGATCAAGATCAACAGC | Hypothetical lipoprotein | 157 | 60 | 100.5 |
SPICI04_139 | ACAACGAAGGTACATCATTAACAAC and TTTGCTGGAGTAATTTGAACATAAAC | Spiralin | 80 | 60 | 100.8 |
SPICI05_014 | CCGGTATAACCTTTTGTCAC and AATTAGTTCAACGCTTTGAG | Hypothetical lipoprotein | 138 | 60 | 96.1 |
SPICI06_025 | CTAATACACAACAACCGCCC and CTTTACACCAGATGTATCGTG | Hypothetical lipoprotein | 161 | 60 | 103.1 |
SPICI07_030 | CTTCCCGTACTTACTAACG and ATACTAAAGATTTGGGAGGC | Hypothetical lipoprotein | 160 | 52 | 108.8 |
SPICI09_027 | TTGCCCGCTAATATCTTTTG and TGATTTATGAAATATGATGGTC | Hypothetical lipoprotein | 153 | 52 | 108.8 |
SPICI10_054 | CATCCGGATTTGCAATCAAACC and CAGCGCTTGTCAATTACTGC | Hypothetical lipoprotein | 505 | 60 | 97.8 |
SPICI10_055 | GGTGACGAAGGAATTGATGC and CCTGCGCTCATTGTAACATC | Hypothetical lipoprotein | 206 | 60 | 96.1 |
SPICI11_003 | GTGCAATTAAAAGTAGG and GTGCAATTAAAAGTAGG | Sc76 | 157 | 52 | 104.6 |
SPICI12_020 | TGCTACTGTTGTTAGTTGTGC and CTCAATTGCAATTTCACCACG | Hypothetical lipoprotein | 200 | 60 | 96.3 |
SPICI12_021 | TGATGCACCACTGAAAATTGG and CGGCAACATCAGGATTATGG | OppA | 536 | 60 | 98.2 |
SPICI12_028 | ACGGTTATTAACACTTTTTAGTG and TCCAAGATCTTGATGACCTTC | Hypothetical lipoprotein | 126 | 60 | 95.6 |
SPICI13_014 | AACCAATTGAACCACCAGAAG and CACAATCATAGACAATTGCTTG | Hypothetical lipoprotein | 228 | 60 | 98.4 |
SPICI16_011 | GTCAATGCCACCGTTTAATGC and AGCACCAGGAATGAAAACAGC | Hypothetical lipoprotein | 535 | 52 | 90.6 |
SPICI20_004 | GAATTATGATGAGGAGAC and AAGTTAAAGTAATTCCTGC | Hypothetical lipoprotein | 191 | 60 | 93.3 |
SPICI20_057 | TTGATGAATCGCTTTCCTATTG and CTTGTGCCATTATTGTATAACC | Hypothetical lipoprotein | 360 | 60 | 95.5 |
SPICI20_065 | GTGAAGGCACAGTTACTCC and GCTGAGCCAGAACTTGAAC | Hypothetical lipoprotein | 562 | 60 | 100.3 |
SPICI20_066 | TTAAGCGCTATGGTAGTGGC and ATACCTGGTGTTGCTGTGTC | Hypothetical lipoprotein | 445 | 60 | 91.8 |