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Fig. 1 | BMC Microbiology

Fig. 1

From: Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromate

Fig. 1

ChrC (a) and ChrF (b) were overexpressed with carboxy-terminal 6-histidine tags in E. coli BL21 (DE3). Lane 1, DNA ladder; lane 2, whole-cell extract from uninduced cells containing pET30a expressing the proteins; lane 2, whole-cell extract from induced cells containing pET30a expressing the proteins; lane 3, purified ChrC-His6 (a) and ChrF-His6 (b) by Ni2+ nitrilotriacetic acid affinity chromatography

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