Fig. 2

HtpG is required for resistance to λvir at 30 °C and 37 °C to prevent Cas3 instability. a E. coli cell lawns of strain Δhns Δcas1 + λc + λT3 (IIB1040) transformed with pCas3 and pHtpG expressing plasmids and empty vector controls were infected with phage dilutions (from 10⁰ to 10⁻⁸) and incubated at 37 °C. Bars represent average and SD of the number of plaque forming units (PFUs) per ml from three independent experiments. b E. coli cell lawns of strains Δhns Δcas1 + λc + λT3 (IIB1040) and ΔhtpG Δhns Δcas1 + λc + λT3 (IIB1066) and IIB1066 transformed with pCas3 and pHtpG expressing plasmids, were infected with phage dilutions (from 10⁰ to 10⁻⁸) and incubated at either 30 °C or 37 °C. Bars represent average and SD of the number of plaque forming units (PFUs) per ml from three independent experiments