Primer | Sequence (5′ to 3′, underlined sequences are engineered restriction sites) | Description |
---|---|---|
pw30 | GTATTAGAGCTCATCGCACTGCTTCTTGAGGT | For tdaA insertional mutation, forward, with SacI site |
pw31 | CGACTATCTAGAGATGATTGGGTCCTTTGCAC | For tdaA insertional mutation, reverse, with XbaI site |
pw32 | GTATTAGAGCTCAGCAGCCATGAATAGCCTGT | For tdaB insertional mutation, forward, with SacI site |
pw33 | CGACTATCTAGAGGGTATCGGATTTCGGATTT | For tdaB insertional mutation, reverse, with XbaI site |
pw36 | GTATTAGAGCTCATCTTTGGCTCCATCGACAT | For tdbD insertional mutation, forward, with SacI site |
pw37 | CGACTATCTAGAGCACATTGTTGGGAAACTGA | For tdbD insertional mutation, reverse, with XbaI site |
pw108 | GAAGAGCTCGGACGACTATGTGATTGGTCAGGC | For clpX insertional mutation, forward, with SacI site |
pw109 | GGGTCTAGACGACGTTATATTCCGACGCCTGCA | For clpX insertional mutation, reverse, with XbaI site |
pw153 | GTATTAGAGCTCGAGCATAACCGCTTTGCCCGCCGCCCA | For exoP insertional mutation, forward, with SacI site |
pw154 | CGACTATCTAGACCATGCTGAGTGCAAGGTTGACGGCGG | For exoP insertional mutation, reverse, with XbaI site |
pw127 | GCATTAGAGCTCGTCAGATTGGCCGAAGCCCCTTTT | For clpX in trans complement, forward, with SacI site |
pw128 | CGGCTATCTAGACGAACTCACCACCTGAGGAGATACGT | For clpX in trans complement, reverse, with XbaI site |
pw166 | GTATTAGAGCTCCCCGTCCGATGTGTCAAAATAGGT | For exoP in trans complement, forward, with SacI site |
pw165 | CGTCTTTCTAGAGGTGCCTGCGGTCATCACCATGAC | For exoP in trans complement, reverse, with XbaI site |
pwGFP-F | GCGGTACATATGTAAGGAGGAAAAACATATG | For amplification of gfp ORF, forward, with NdeI site |
pwGFP-R | CTATATGGATCCCAGATCTATTTGTATAGTTCATCCA | For amplification of gfp ORF, reverse, with BamHI site |
Pm113 | GGTACCTGTCTGTCGCCTCTTGT | For amplification of PflaB, forward, with KpnI site |
Pm114 | GGTACCATATCATTCCTCCATGAT | For amplification of PflaB, forward, with KpnI site |
pwmO-F | GCGGTACATATGATGGTGAGCAAGGGCGAGGAGAAT | For amplification of ofp ORF, forward, with NdeI site |
pwmO-R | CTATATGGATCCCTTGTACAGCTCGTCCATGCCGCC | For amplification of ofp ORF, reverse, with BamHI site |