Strains or plasmids | Description | Resistance | Reference |
---|---|---|---|
P. inhibens | |||
S4 | Previously Phaeobacter sp. S4; wild type isolate from the inner shell of oysters | Â | Karim et al., 2013 |
S4Sm | Spontaneous Smr mutant of S4 | Smr | this study |
WZ10 | clpX insertional mutant of S4Sm | Smr Cmr | this study |
WZ11 | clpX+, clpX in trans complement of WZ10 | Smr Cmr Apr | this study |
WZ20 | exoP insertional mutant of S4Sm | Smr Cmr | this study |
WZ21 | exoP+, exoP in trans complement of WZ20 | Smr Cmr Apr | this study |
WZ02 | S4Sm (pRhokHi-2-ofp) | Smr Cmr Kmr | this study |
WZ12 | clpX, WZ10 (pRhokHi-2-ofp) | Smr Cmr Kmr | this study |
WZ22 | exoP, WZ20 (pRhokHi-2-ofp) | Smr Cmr Kmr | this study |
V. tubiashii | |||
RE22 | Wild type isolate from oyster larvae | Â | Estes et al., 2004 |
RE22Sm | Spontaneous Smr mutant of RE22 | Smr | this study |
WZ103 | RE22Sm (pRhokHi-2-gfp) | Smr Apr | this study |
V. anguillarum | |||
NB10 | Wild type, serotype O1, clinical isolate from the Gulf of Bothnia | Â | Norqvist et al., 1989 |
NB10Sm | Spontaneous Smr mutant of NB10 | Smr | this study |
WZ203 | NB10Sm (pSUP202P-PflaB-gfp) | Smr Apr Tetr | this study |
R. crassostreae | |||
CV919-312T | Wild type isolate from a JOD-affected oyster | Â | Boettcher et al., 1999 |
CV919Sm | Spontaneous Smr mutant of CV919-312Â T | Smr | this study |
E. coli | |||
Sm10 | thi thr leu tonA lacY supE recA RP4-2 Tc::Mu::Km (λpir) | Kmr | Simon et al., 1983 |
S100 | Sm10 harboring pNQ705-1 | Â | this study |
WQ10 | Sm10 harboring pNQ705-clpX | Â | this study |
WQ20 | Sm10 harboring pNQ705-exoP | Â | this study |
WB01 | Sm10 harboring pBBR1MCS4 | Â | this study |
WB11 | Sm10 harboring pBBR1MCS4-clpX | Â | this study |
WB21 | Sm10 harboring pBBR1MCS4-exoP | Â | this study |
S122 | Sm10 harboring pSUP202P-gfp(ORF) | Â | this study |
S136 | Sm10 harboring pSUP202P-PflaB-gfp | Â | this study |
W900 | Sm10 harboring pRhokHi-2-FbFP | Â | this study |
WR03 | Sm10 harboring pRhokHi-2-gfp | Â | this study |
WR02 | Sm10 harboring pRhokHi-2-ofp | Â | this study |
W901 | Sm10 harboring pmOrange | Â | this study |
Plasmids | |||
pNQ705-1 | Cmr; suicide vector with R6K origin | Â | Mcgee, 1996 |
pNQ705-clpX | Cmr; derivative from pNQ705-1 for clpX insertional mutant | Â | this study |
pNQ705-exoP | Cmr; derivative from pNQ705-1 for exoP insertional mutant | Â | this study |
pBBR1MCS4 | Apr; derivative from pBBR1MCS (a broad-host-range cloning vector) | Â | Kovach et al., 1995 |
pBBR1MCS4-clpX | Apr; derivative from pBBR1MCS4 for clpX in trans complement | Â | this study |
pBBR1MCS4-exoP | Apr; derivative from pBBR1MCS4 for exoP in trans complement | Â | this study |
pBS(gfp)-Pcampy | Template for gfp ORF PCR amplification | Â | Eggers et al., 2004 |
pCE320(gfp)-PflaB | Template for PflaB PCR amplification | Â | Eggers et al., 2004 |
pSUP202P | Apr Cmr Tcr; broad host shuttle vector | Â | Simon et al., 1983 |
pSUP202P-gfp(ORF) | Apr Tcr; derivative from pSUP202 for GFP tagging | Â | this study |
pSUP202P-PflaB-gfp | Apr Tcr; derivative from pSUP202 for GFP tagging | Â | this study |
pRhokHi-2-FbFP | Cmr Kmr; derivative from pBBR1MCS (a broad-host-range cloning vector) with promoter PaphII | Â | Piekarski et al., 2009 |
pRhokHi-2-gfp | Cmr Kmr; derivative from pRhokHi-2-FbFP with gfp under the control of PaphII | Â | this study |
pmOrange | Template for ofp ORF PCR amplification | Â | Clontech Laboratories, Inc. |
pRhokHi-2-ofp | CmrKmr; derivative from pRhokHi-2-FbFP with ofp under the control of PaphII | Â | this study |