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Table 2 Strains isolated from different environments, their molecular identification and biosurfactant production and other characteristics

From: Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production

Strains

Isolation media

Identification (% identity) – accession number a

BOX group

E24-Hex

E24-Ker

DC

OD (cm)

H

sfp

AMS

(EI %)

(EI %)

1 day

15 days

30 days

1 day

15 days

30 days

Vermelha Lagoon

              

 VM 1.0

MB

Bacillus aquimaris (99) - JF411268.1

 

27 ± 2.3

-

-

-

-

-

-

-

-

-

-

 VM 1.1

MB

B. subterraneus (100) - KJ722431.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 2.0

MB

B. infantis (99) - KJ911913.1

 

-

-

-

-

-

-

-

-

-

+

+

 VM 2.1

MB

B. infantis (100) - KJ911913.1

 

10 ± 0

8 ± 0

-

24 ± 5.7

16 ± 8

12 ± 5.7

+

-

-

+

+

 VM 3.0

MB

Halobacillus trueperi (99) - KJ009504.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 3.1

MB

B. vietnamensis (100) - JF411279.1

14

38 ± 6.9

38 ± 6.9

38 ± 6.9

-

-

-

+

-

-

-

+

 VM 4.0

MB

H. mangrovi (99) - JQ799099.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 5.0

MB

B. berkeleyi (99) - NR109459.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 5.1

MB

B. berkeleyi (99) - NR109459.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 6.0

MB

B. infantis (99) - KJ911913.1

15

19 ± 2.3

15 ± 1.2

15 ± 1.2

34 ± 2.8

27 ± 9.2

13 ± 8.3

-

-

-

+

+

 VM 6.1

MB

B. infantis (99) - KJ911913.1

16

47 ± 5.8

24 ± 6.9

21 ± 6.1

8 ± 83.5

6 ± 0

-

-

-

-

-

-

 VM 7.0

MB

H. trueperi (99) - KJ009504.1

 

-

-

-

-

-

-

-

-

-

+

-

 VM 7.1

MB

Thalassobacillus hwangdonensis (100) - NR104552.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 8.0

MB

B. infantis (99) - KJ911913.1

9

19 ± 1.2

19 ± 1.2

15 ± 1.2

30 ± 2.8

17 ± 1.2

11 ± 1.2

-

-

-

-

-

 VM 8.1

MB

B. infantis (99) - KJ911913.1

 

21 ± 1.2

21 ± 1.2

21 ± 1.2

-

-

-

+

-

+

-

-

 VM 9.0

MB

H. mangrove (99) - JQ799099.1

 

-

-

-

-

-

-

-

-

-

+

-

 VM 10

TSB + 3.5 % NaCl

B. subterraneus (99) - KJ722431.1

 

-

-

-

-

-

-

-

-

-

+

-

 VM 20

TSB + 3.5 % NaCl

B. infantis (99) - KJ911913.1

17

18 ± 3.5

12 ± 4

12 ± 4

32 ± 5.7

-

-

-

-

-

-

+

 VM 30

TSB

B. infantis (99) - KJ911913.1

4

33 ± 2.3

33 ± 2.3

30 ± 0

36 ± 2.8

28 ± 5.7

20 ± 5.7

+

-

-

-

-

 VM 40

TSB

B. infantis (99) - KJ911913.1

10

23 ± 2.3

23 ± 2.3

23 ± 2.3

36 ± 2.8

31 ± 9.5

31 ± 9.5

-

-

-

-

-

 VM 41

TSB

B. infantis (99) - KM077280.1

4

17 ± 6.1

16 ± 7.2

16 ± 7.2

32 ± 2.8

24 ± 3.5

22 ± 3.5

-

-

-

-

-

 VM 50

TSB

B. licheniformis (100) - LN774355.1

12

-

-

-

31 ± 2.3

-

-

-

-

+

-

+

 VM 60

TSB

Paenibacillus campinasensis (99) - JF830004.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 70

TSB + 7 % NaCl

T. devorans (100) - JQ799100.1

 

-

-

-

-

-

-

-

-

-

-

-

 VM 80

TSB + 3.5 % NaCl

B. infantis (99) - KM077280.1

18

13 ± 1.2

13 ± 1.2

13 ± 1.2

38 ± 3.5

29 ± 8.3

19 ± 8.1

+

-

-

-

-

 VM 81

TSB + 3.5 % NaCl

B. infantis (99) - KJ911913.1

4

54 ± 7.2

47 ± 4.6

42 ± 3.5

27 ± 5.8

25 ± 4.6

19 ± 2.3

-

-

-

-

-

Dois Rios Beach

              

 DR 1.0

TSB

B. niacini (99) - KJ542777.1

 

-

-

-

-

-

-

-

-

-

-

-

 DR 2.0

TSB

B. asahii (99) - JN084129.1

7

-

-

-

32 ± 2.8

19 ± 9

14 ± 3.5

-

-

-

-

-

 DR 3.0

MB

Fictibacillus barbaricus (99) - KJ575018.1

 

-

-

-

-

-

-

-

-

-

-

-

 DR 4.0

MB

F. barbaricus (99) - KC778367.1

6

63 ± 2.3

63 ± 2.3

61 ± 1.2

-

-

-

-

-

+

-

-

Massambaba Beach

              

 MA 1.0

MB

B. subtilis (99) - KJ668821.1

1

-

-

-

56 ± 6.4

46 ± 7.8

43 ± 4.2

+

2.3

+

+

-

 MA 2.0

MB

B. aquimaris (99) - EU624438.1

8

-

-

-

56 ± 2.1

31 ± 5.2

30 ± 3.5

-

-

-

+

-

 MA 2.1

MB

B. aquimaris (99) - KM677908.1

 

-

-

-

-

-

-

-

-

+

+

-

 MA 3.0

MB

B. subtilis (100) - KP100527.1

1

-

-

-

54 ± 3

46 ± 4.6

46 ± 4.6

+

2

+

+

+

 MA 3.1

MB

B. subtilis (100) - KP100527.1

3

28 ± 4.9

25 ± 1.7

24 ± 1.2

45 ± 0

44 ± 1.7

40 ± 5.2

-

1.5

+

+

+

 MA 5.0

MB

B. subtilis (100) - KP100527.1

2

34 ± 6

34 ± 6

32 ± 3.5

-

-

-

+

2.5

+

+

-

 MA 5.1

MB

B. tequilensis (99) - KC851826.1

1

48 ± 5.5

26 ± 2.9

26 ± 2.9

27 ± 1.7

17 ± 6

17 ± 6

+

1.5

+

+

-

 MA 6.0

MB

B. aquimaris (100) - KM677908.1

 

-

-

-

-

-

-

-

-

-

-

-

 MA 6.1

MB

B. aquimaris (99) - KM677908.1

 

14 ± 0

13 ± 1.7

10 ± 3.5

-

-

-

-

-

+

+

-

 MA 7.0

MB

B. subtilis (99) - KP100527.1

3

26 ± 2.9

-

-

48 ± 4.2

41 ± 13.8

35 ± 9.2

+

1.7

+

+

-

 MA 7.1

MB

B. subtilis (99) - KJ668821.1

1

36 ± 3.5

30 ± 3.5

30 ± 3.5

13 ± 1.7

-

-

+

3

+

+

-

 MA 8.0

TSB

B. subtilis (99) - KP100527.1

3

30 ± 2.1

27 ± 1.7

25 ± 2.9

18 ± 4.6

14 ± 7.9

11 ± 5.2

+

1.7

+

+

-

 MA 9.0

MB

B. aquimaris (100) -KM677908.1

 

-

-

-

-

-

-

-

-

-

+

-

 MA 9.1

MB

B. aquimaris (100) -KM677908.1

 

-

-

-

-

-

-

-

-

+

+

-

 MA 10.0

MB

B. subtilis (100) -KP100527.1

1

9 ± 1.7

8 ± 0

-

45 ± 4.6

44 ± 3.5

35 ± 1.7

+

1.9

+

+

-

 MA 10.1

MB

B. subtilis (100) -KP100527.1

1

-

-

-

55 ± 1.7

49 ± 7.1

46 ± 4.6

+

1.8

+

+

-

 MA 11.0

MB

B. subtilis (99) -KP100527.1

3

-

-

-

46 ± 2.9

38 ± 8.7

34 ± 6

+

3.1

+

+

-

 MA 11.1

MB

B. subtilis (99) -KP100527.1

3

-

-

-

44 ± 1.2

44 ± 1.7

30 ± 1.7

+

2

+

-

-

 MA 12.0

MB

B. subtilis (99) -KF601955.1

 

-

-

-

14 ± 0

7 ± 4

7 ± 4

+

2.5

+

+

-

 MA 12.1

MB

B. subtilis (100) -KP100527.1

2

-

-

-

54 ± 3

50 ± 3.5

44 ± 1.7

+

1.6

+

+

-

 MA 13.0

MB

B. subtilis (100) KP100527.1

2

-

-

-

51 ± 0

25 ± 5.5

19 ± 6.9

+

2.0

+

+

-

 MA 13.1

MB

B. tequilensis (99) - KC851826.1

1

4 ± 1.2

-

-

53 ± 4.6

48 ± 5.5

44 ± 1.7

+

2.7

+

+

-

 MA 14.0

MB

B. subtilis (100) KP100527.1

3

8 ± 0

8 ± 0

-

46 ± 2.9

32 ± 3.5

28 ± 0

+

2.5

+

+

-

 MA 14.1

MB

B. subtilis (100) KP100527.1

3

-

-

-

53 ± 1.7

45 ± 0

44 ± 1.7

+

1.1

+

+

-

 MA 15.0

TSB + 3.5 % NaCl

B. subtilis (100) KP100527.1

1

36 ± 7.5

31 ± 6

31 ± 6

18 ± 3.5

12 ± 4.6

12 ± 4.6

+

2

+

+

-

 MA 15.1

TSB + 3.5 % NaCl

B. licheniformis (100) - KP052704.1

11

32 ± 3.5

25 ± 2.9

23 ± 0

22 ± 4.6

22 ± 4.6

23 ± 5.2

+

1.6

+

+

+

 MA 16.0

TSB + 3.5 % NaCl

B. tequilensis (99) - KC851826.1

1

20 ± 0

20 ± 0

19 ± 1.7

51 ± 4.2

48 ± 4.2

43 ± 4.2

+

2.2

+

+

-

 MA 16.1

TSB + 3.5 % NaCl

B. subtilis subsp. subtilis (99) – KM972669.1

3

12 ± 3.5

12 ± 3.5

11 ± 4.6

46 ± 4.6

26 ± 7.1

18 ± 6.9

+

2.6

+

+

-

Abraão Beach

              

 AB 1.0

TSB + 3.5 % NaCl

B. subtilis subsp. subtilis (99) – KM972669.1

 

-

-

-

-

-

-

+

1.8

+

+

-

 AB 1.1

TSB + 3.5 % NaCl

B. subtilis subsp. subtilis (99) – KM972669.1

3

14 ± 0

10 ± 3.5

10 ± 3.5

51 ± 0

37 ± 4.2

29 ± 3.5

+

2.5

+

+

-

 AB 2.0

TSB + 3.5 % NaCl

B. subtilis (100) KP100527.1

1

46 ± 2.9

32 ± 1.7

28 ± 0

59 ± 1.7

53 ± 3.5

53 ± 3.5

+

2

+

+

+

 AB 3.0

TSB + 3.5 % NaCl

B. aquimaris (100) - KM516787.1

 

-

-

-

-

-

-

-

-

-

-

-

 AB 4.0

TSB + 3.5 % NaCl

B. aquimaris (99) - KM516787.1

 

-

-

-

19 ± 1.7

-

-

-

-

-

-

-

Saltern 1

              

 SA1 1

TSB

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

+

+

-

 SA1 2

TSB + 7 % NaCl

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

+

-

-

 SA1 3

TSB + 7 % NaCl

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA1 4

TSB + 7 % NaCl

F. barbaricus (98) -KJ575018.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA1 5

MB

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA1 6

MB

B. oceanisediminis (100) - KM873030.1

 

-

-

-

-

-

-

-

-

+

-

-

 SA1 7

MB

B. firmus (99) - KP119100.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA1 8

MB

B. firmus (99) - KF886286.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA1 10

MB

B. firmus (99) - KP119100.1

2

32 ± 3.5

-

-

49 ± 1.7

-

-

-

-

+

-

-

 SA1 11

MB

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

+

-

-

 SA1 12

MB

F. barbaricus (99) - KJ831620.1

 

-

-

-

-

-

-

-

-

+

-

-

 SA1 13

MB

F. barbaricus (100) - KJ831620.1

 

-

-

-

-

-

-

-

-

+

-

-

 SA1 14

MB

B. firmus (100) -KP119100.1

2

-

-

-

51 ± 7.4

-

-

-

-

+

-

-

 SA1 15

MB

B. firmus (100) - KJ528874.1

2

-

-

-

52 ± 7.1

-

-

-

-

+

-

-

 SA1 16

MB

B. firmus (99) - KF886286.1

2

38 ± 3.5

38 ± 3.5

14 ± 0

52 ± 1.7

13 ± 1.7

-

-

-

-

-

-

 SA1 17

MB

B. firmus (99) - KP119100.1

2

43 ± 2.9

-

-

48 ± 0

17 ± 5.2

-

-

-

-

-

-

 SA1 18

TSB + 7 % NaCl

B. firmus (99) - KP119100.1

2

41 ± 1.7

14 ± 0

-

46 ± 2.9

15 ± 1.7

-

-

-

-

-

-

 SA1 19

TSB + 7 % NaCl

B. niabensis (99) -KF861607.1

 

-

-

-

-

-

-

-

-

-

-

-

Saltern 2

              

 SA2 1

MB

B. oceanisediminis (100) - KM873030.1

5

39 ± 1.7

14 ± 0

-

51 ± 4.2

-

-

-

-

-

-

-

 SA2 2

MB

B. firmus (100) - KP119100.1

5

36 ± 1.7

7 ± 4

7 ± 4

50 ± 6.4

-

-

-

-

-

-

-

 SA2 3

MB

B. firmus (100) - KP119100.1

2

-

-

-

65 ± 2.9

44 ± 10

38 ± 6.9

-

-

-

-

-

 SA2 4

MB

B. firmus (100) - KP119100.1

5

-

-

-

51 ± 7.4

43 ± 4.6

42 ± 3.5

-

-

-

-

-

 SA2 5

MB

B. firmus (100) - KP119100.1

2

-

-

-

51 ± 4.2

-

-

-

-

-

-

-

 SA2 6

MB

B. firmus (100) - KP119100.1

 

-

-

-

-

-

-

-

-

-

-

-

 SA2 7

MB

B. firmus (100) - KP119100.1

2

-

-

-

51 ± 8.5

38 ± 7

-

-

-

-

-

-

 SA2 8

MB

B. oceanisediminis (100) - KM873030.1

2

45

-

-

-

-

-

-

-

-

-

-

 SA2 9

MB

B. oceanisediminis (99) - KM873030.1

2

23 ± 8.1

-

-

51 ± 7.4

48 ± 4.6

-

-

-

-

-

-

Saltern 2 (mud)

              

 LS 1

TSB + 7 % NaCl

B. horikoshii (100) - KJ722424.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 2

TSB + 7 % NaCl

B. horikoshii (100) - KJ722424.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 3

TSB + 7 % NaCl

B. horikoshii (100) - KJ722424.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 4

TSB + 7 % NaCl

B. horikoshii (100) - KJ722424.1

 

-

-

-

-

-

-

-

-

+

-

-

 LS 5

TSB + 7 % NaCl

B. horikoshii (99) - KJ719363.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 6

MB

B. vietnamensis (99) - JF411279.1

 

-

-

-

-

-

-

-

-

+

-

-

 LS 7

MB

B. vietnamensis (99) - JF411279.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 8

MB

B. vietnamensis (100) - JF411279.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 10

MB

B. vitnamensis (99) - JF411279.1

 

-

-

-

-

-

-

-

-

+

-

-

 LS 11

MB

B. foraminis (97) - KF724906.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 12

TSB

B. foraminis (99) - KF724906.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 13

TSB

Paenisporosarcina indica (99) - NR108473.1

13

-

-

-

59 ± 7.1

38 ± 7

-

-

-

-

-

-

 LS 14

TSB

P. quisquiliarum (99) - JF309238.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 15

TSB

P. quisquiliarum (99) - JF309238.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 16

TSB

B. licheniformis (100) - LN774355.1

 

-

-

-

-

-

-

-

-

-

-

+

 LS 17

TSB + 3.5 % NaCl

B. aquimaris (99) - JF411268.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 18

TSB + 3.5 % NaCl

P. indica (99) - NR108473.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 19

TSB + 3.5 % NaCl

B. thioparans (99) - KJ911915.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 20

TSB + 3.5 % NaCl

B. foraminis (99) -KF724906.1

 

-

-

-

-

-

-

-

-

-

-

-

 LS 21

TSB + 3.5 % NaCl

B. foraminis (99) -KF724906.1

 

-

-

-

-

-

-

-

-

-

-

-

  1. a First hit in GenBank with a known species; EI emulsification index, Hex hexadecane, Ker kerosene, DO drop collapse, OD oil displacement, H hemolysis, sfp presence of sfp gene, AMS production of an antimicrobial substance. (−) negative results. Strains in bold are those showing emulsification values higher than 30 %
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BMC Microbiology

ISSN: 1471-2180

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