Skip to main content
Fig. 1 | BMC Microbiology

Fig. 1

From: Chromosomal integration vectors allowing flexible expression of foreign genes in Campylobacter jejuni

Fig. 1

Construction of expression vectors for integration onto the C. jejuni chromosome. Construction of the pCJC series of plasmids for integration in the C. jejuni 11168 pseudogene Cj0223. A fragment of Cj0223 cloned into pUC18 is interrupted by a chloramphenicol resistance cassette (cat) at a unique SpeI site. Promoter regions with Shine-Dalgarno (SD) sites were cloned into the XhoI site followed by insertion of gfp at the NdeI site. Three promoter regions were used: H. pylori ureI to create pCJC2, H. pullorum flaA to create pCJC3 and C. jejuni porA to create pCJC4

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us