Table 1 Strains and plasmids used in this study

 DH5 α

Φ901acZΔm15, recA1

Invitrogen

  Xac 29-1

Wild-type

This lab

  Xac 29-1/pUGP_carAB

Xac 29–1 carrying pUGP_carAB

This work

  ΔcarA

A carA knock-out mutant of strain Xac 29-1

[9]

  ΔcarB

A carB knock-out mutant of strain Xac 29-1

[9]

 CΔcarA

Gm^r, ΔcarB harboring pBBR1MCS-5 expressing carA gene under wxacO promoter

[9]

 CΔcarB

Gm^r, ΔcarB harboring pBBR1MCS-5 expressing carB gene under wxacO promoter

[9]

 pUFR034

Km^r, IncW, Mob(p), Mob ⁺ , LacZa ⁺, PK2 replicon, cosmid

[29]

 pUGP_carAB

Km^r, carAB operon promoter and gusA gene fused in pUFR034 vector

This work