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Fig. 2 | BMC Microbiology

Fig. 2

From: Molecular study on the carAB operon reveals that carB gene is required for swimming and biofilm formation in Xanthomonas citri subsp. citri

Fig. 2

Analysis of carAB operon promoter and its activity. a Nucleotide sequence of carAB operon promoter region. The promoter sequence was shaded in grey colour, and the −35 and −10 promoter elements were overlined. Dotted-line arrows indicated the forward and reverse primers for promoter cloning. The transcription initiation was shown by a black arrow. b The promoter-monitored GUS activities on MMX plates. The applied substrates uracil, glutamic acid and arginine were indicated by the first letter as U, G and A. U, uracil; G, glutamic acid; A, arginine; UA, uracil and arginine; UG, uracil and glutamic acid; GA, glutamic acid and arginine; UGA, uracil, glutamic acid and arginine. c Quantification of GUS activities in MMX liquid media. The experiment was repeated three times, and similar results were obtained. The asterisks in horizontal data column indicate significant differences at P = 0.01 by t test

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BMC Microbiology

ISSN: 1471-2180

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