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Table 2 Characteristics of aEPEC strains positive for EAF plasmid-encoded genes either by PCR or hybridization assay

From: Genetic analysis of enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid reveals a new deletion within the EAF probe sequence among O119 typical EPEC strains

Strain

Serotype

Source

Presence or absence of:

IS element upstream of per

Presence or absence of:

HEp-2 adhesion pattern

bfpA (probe)

bfpA (PCR)

bfpA-IS66

trcP (probe)

per (probe)

perA (PCR)

EAF (PCR)

orf35–36 (probe)

orf61–62 (probe)

A103

O9:HND

Patient

+

IS1294

+

+

A148

O37:NM

Patient

IS1294

+

+

A141

O49:HND

Patient

IS1294

+

+

+

A69

O55:NM

Patient

+

+

+

IS1294

+

+

LAL

A152

O96:NM

Control

IS1294

+

+

A144

O98:HND

Patient

+

IS1294

+

+

+

A140

O108:HND

Control

+

+

IS1294

+

+

LAL

A60

O119:H2

Patient

+

+

IS1294

+

+

LAL

A62

O119:H2

Patient

+

+

IS1294

+

+

LAL

A66

O119:H2

Control

+

+

IS1294

+

+

LAL

A67

O119:H2

Patient

+

+

IS1294

+

+

LAL

A75

O119:HND

Patient

+

+

IS1294

+

+

LAL

A90

O119:HND

Patient

+

+

IS1294

+

+

LA

A111

O119:HND

Patient

+

+

IS1294

+

+

LAL

A127

O119:HND

Patient

+

+

IS1294

+

+

LAL

A131

O119:HND

Patient

+

+

IS1294

+

+

LAL

A97

O128:HND

Patient

IS1294

+

+

A126

O128:NM

Patient

IS1294

+

+

A11

O142:NM

Patient

+

+

+

IS1294

+

+

+

LA

A129

O142:HND

Patient

+

+

+

IS1294

+

+

+

LAL

A124

O157:NM

Patient

IS1294

+

+

A95

ONT:H21

Patient

+

+

+

IS1294

+

+

LAL

A65

ONT:NM

Control

+

+

IS1294

+

+

LAL

A74

ONT:NM

Patient

+

+

IS1294

+

+

LAL

A63

ONT:HND

Patient

+

+

IS1294

+

+

+

+

LAL

A64

ONT:HND

Patient

+

+

IS1294

+

+

+

+

LAL

A78

ONT:HND

Patient

+

+

IS1294

+

+

+

LAL

A146

ONT:HND

Control

+

+

IS1294

+

+

+

LAL

A57

ONT:HND

Patient

IS1294

+

+

A16

ONT:HND

Control

IS1294

+

+

+

A118

ONT:HND

Patient

IS1294

+

+

A136

ONT:HND

Patient

IS1294

+

+

+

LAL

  1. ND Nondetermined; NM Nonmotile; LA Localized adherence patter; LAL LA-like pattern