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Table 1 Screening of bacterial isolates for cholesterol (100 mg/dl) decomposition in liquid medium and total viable count on solid medium (Log N)

From: Purification and characterisation of the extracellular cholesterol oxidase enzyme from Enterococcus hirae

Isolate source

Isolate No.

Residual cholesterol (mg/dl)

% of decomposition

Dry weight (mg/50 ml)

Log N

Total isolates

Raw milk (RM) (n = 20)

1

64.80

35.2

13.3

6.38

7

30

84.86

15.14

5.70

36

69.89

30.11

11.4

38

49.74

50.26

19.02

50

59.80

40.2

15.22

Mean SD

 

*65.82a

*34.18a

*12.92a

  
 

12.98

12.99

4.92

Whey (W) (n = 20)

2

84.90

15.10

5.70

6.28

14

8

90.30

9.70

3.80

16

59.72

40.28

15.20

24

84.86

15.14

5.70

42

45.20

54.80

20.93

Mean SD

 

*72.99a

*27.00a

*10.26a

  
 

19.65

19.56

7.44

Yogur (Y) (n = 20)

3

74.86

25.14

9.50

6.40

9

10

64.89

35.11

13.30

18

64.39

35.61

13.30

28

39.92

60.08

22.82

44

79.73

20.27

7.60

Mean SD

 

*64.75*a

*35.24a

13.30a

  
 

15.35

15.35

5.86

Milk (M) (n = 20)

4

69.75

30.25

11.40

6.37

9

7

49.70

50.30

19.00

22

54.63

45.37

17.12

31

24.70

75.30

28.54

Mean SD

 

*49.69a

*50.30a

*19.01a

  
 

18.72

18.72

7.10

Cheese (Ch) (n = 20)

12

79.50

20.50

7.60

6.35

11

14

30.20

69.80

26.63

34

54.75

45.25

17.12

40

75.13

24.87

9.51

46

54.66

45.34

17.12

Mean SD

24

*58.84a

*41.15a

*15.59a

*6.35a 0.041

50

 

19.66

19.66

7.53

  1. n=20 : is the number of each sample
  2. *, aDifferent superscripted letters indicate that no significant differences (P ≤ 0.05) among the observed values within columns (Duncan’s multiple range test)