Fig. 5
mRNA expression levels of the T. cruzi life cycle stages (trypomastigotes, amastigotes, epimastigotes, and metacyclic trypomastigotes) determined by microarray analysis [45]. Six hybridizations were performed and consisted of three dye-swap experiments from three independent samples (biological replicates). In each case, the experimental sample was from a single life-cycle stage and the control sample was an equal mixture of all four life-cycle stages [45]. Expression values for 2-channel microarray experiments are shown as log ratios (M = log2 Cy5/Cy3) [45]. The numbers refer to different mRNAs: 1- TcCLB.510879.130 (hypothetical protein); 2- TcCLB.503975.50 (hypothetical protein); 3- TcCLB.509599.130 (serine/threonine protein kinase); 4- TcCLB.504163.60 (hypothetical protein); 5- TcCLB.509455.114 (hypothetical protein); 6- TcCLB.504005.54 (hypothetical protein); 7- TcCLB.508719.70 (flagellum targeting protein Kharon 1); 8- TcCLB.511365.80 (mitochondrial carrier protein); 9- TcCLB.506825.200 (hypothetical protein); 10- TcCLB.506529.324 (hypothetical protein); 11- TcCLB.506529.508 (glucose-6-phosphate isomerase); 12- TcCLB.510535.100 (cysteine C peptidase CPC); 13- TcCLB.511801.60 (mitochondrial DEAD-box helicase)