Figure 6
From: Characterization of motility and piliation in pathogenic Neisseria
Analysis of Tfp-mediated motility using TIRF microscopy. Live bacteria were stained with the Dylight™488 NHS-ester and monitored using time-lapse microscopy. (A) Representative frames of N. gonorrhoeae strain FA1090 motility on a poly-D-lysine-coated glass surface. The arrows mark a short repetitively retracting filament. The scale bars correspond to 5 μm. (B) Representative frames of N. meningitidis strain FAM20 motility on a poly-D-lysine-coated glass surface. Several long filaments contribute to the movement of the bacterial cell. The red lines indicate the track of the bacteria. The numbers indicate the time in seconds. For videos, see supplemental Video S1 and Video S2. (C and D) The color-coded bacterial tracks were generated from Videos S1 (C; N. gonorrhoeae) and S2 (D; N. meningitidis). The colors indicate point velocities and are explained in the figure inset. The scale bars correspond to 1 μm.