Table 1 Bacterial strains and plasmids used in this study
Bacterial strain or plasmid | Description a | Source or reference | |
|---|---|---|---|
A. salmonicida | |||
| Â | LFI1238 | Wild type, isolated from Atlantic cod | [11] |
|  | ΔlitR | LFI1238 containing an in-frame deletion of litR | [10] |
|  | ΔainS | LFI1238 containing an in-frame deletion of ainS | This study |
|  | ΔainS/luxI ˉ | ΔainS with insertional disruption of luxI; Cmr | This study |
|  | luxI ˉ | LFI1238 with insertional disruption of luxI; Cmr | This study |
|  | luxR1ˉ | LFI1238 with insertional disruption of luxR1 gene, Ermr | This study |
|  | luxR2ˉ | LFI1238 with insertional disruption of luxR2 gene, Ermr | This study |
E. coli | |||
|  | S17-1 | Donor strain for conjugation, λ-pir | [42] |
|  | DH5αλ pir | Donor strain for conjugation | [45] |
|  | DH5α | Helper strain for conjugation | Invitrogen |
Plasmids | |||
|  | pDM4 | Cmr; suicide vector with an R6K origin (λ-pir requiring) and sacBR | [43] |
|  | pNQ705 | Cmr; suicide vector with an R6K origin (λ-pir requiring) | [43] |
|  | pEVS122 | Ermr; suicide vector with an R6Kγ oriV, oriT RP4 , lacZα,cosN, loxP, incD | [45] |
| Â | pEVS104 | Helper plasmid | [44] |
|  | pDM4ΔainS | pDM4 containing a fragment of ainS harbouring an internal deletion | This study |
| Â | pNQ705luxI | pNQ705 containing a 245Â bp fragment of luxI | This study |
| Â | pEVSluxR1 | pEVS122 containing a 224Â bp fragment of luxR1 | This study |
| Â | pEVSluxR2 | pEVS122 containing a 280Â bp fragment of luxR2 | This study |