Table 3 Oligonucleotides used in this study
Name | Description | Sequence |
|---|---|---|
YPS1 F | Strain deletion confirmation | 5′-CTTTTATACTGCGCCATGAGTAGTT-3′ |
PMT1 F | Strain deletion confirmation | 5′-CTTCAATTACGCTTTCTACCAACAT-3′ |
PMT2 F | Strain deletion confirmation | 5′-ACATATTATTGGACACGTCGCCC-3′ |
PMT4 F | Strain deletion confirmation | 5′-TTCCTACTTGTCCTTTCTTTCCTTT-3′ |
KANB | Strain deletion confirmation | 5′-CTGCAGCGAGGAGCCGTAAT-3′ |
Loop F | N-ent loop replacement in pRSYPS1 | 5′-[PO4]-GCTCGGATAATCCATACTGTGGT TCTGTGATGGACTGTCAACAATACGG-3′ |
YB E101-F | Mutation D101E | 5′-GGTCCTGGTGGAAACAGGCTCCTCT-3′ |
YB E101-R | Mutation D101E | 5′-AGAGGAGCCTGTTTCCACCAGGACC-3′ |
YB40 | Amplification of pYPS1-DL for gap repair in pssYPS1 | 5′-TGGAAGTGGGCACGCCAC-3′ |
YB43 | Amplification of pYPS1-DL for gap repair in pssYPS1 | 5′-GAACACTATTTCCATACT-3′ |