Figure 6

The number of nuclei in ΔFaTBCA-3 increases under cold stress. Differential interference contrast [DIC] images of nuclei in hyphal tip and middle section of the wild-type GJ33 and ΔFaTBCA-3 were taken after staining with 4',6-diamidino-2-phenylindole (DAPI). Bar = 10 μm. Each strain was incubated in PDB at 25°C for 1 day with a shaker then transferred to static incubation at 4°C for 1 or 3 days.