Skip to main content
Figure 3 | BMC Microbiology

Figure 3

From: An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis

Figure 3

Expression of the rpoE in the Shewanella oneidensis MR-1 activates expression of rpoE , rseA , and degQ . Strain MR-1 carrying the pHERD30T-rpoE plasmid was grown in the presense of L-arabinose (0.05% w/v). The MR-1 strain carrying only empty pHERD30T (with the pBAD promoter) vector was used as control. Note that the transcripts of rpoE in the treatment (the right lanes) include the in trans expression of plasmid-borne rpoE gene, which further enhances the expression of chromosomal loci of rpoE (SO_1342), rseA (SO_1343), and degQ (SO_3942). The transcription of the chromosomal rpoE gene and down-stream cognate anti-sigma factor gene rseA is driven by the same promoter, and an RpoE-recognized promoter for autoregulation has been identified upstream of the rpoE-rseA-rseB-rseC operon. The cells were collected for RNA extraction after 1 hour of induction. A) Transcription of the genes was examined by using semi-quantitative RT-PCR; 16S rRNA gene expression was analyzed and used as the loading control. B) Trace quantity plotting of Figure 3A using ‘Quantity One’ software. The assays were performed in triplicates.

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us