Figure 6
From: CrdR function in a curdlan-producing Agrobacterium sp. ATCC31749 strain
Binding ability of 6 His-tagged crdR to different DNA fragments. A: Different region of the crdP; B: purification of 6 His-tagged crdR; C, D: Binding ability 6 His-tagged crdR to different regions of crdP; E: Binding of 6 His-tagged crdR, to ~450 bp upstream of ATG at different gene coding regions. BM: protein markers, B1: purified His-tagged crdR, B2: supernatant of pBQcrdR/E. coli Bl21; C1. C4, C7, and C10 are 10 μL of 0.5 μM crdP 98, crdP102, crdP142 and crdP mixed with 10 L of 0.5 mg/mL purified 6 His-tagged crdR protein respectively, C2, C5, C8, and C11 are same as C1. C4, C7, and C10, except 6 His-tagged crdR protein was reduced to 0.25 mg/mL; C3, C8, C9, and C12 are same as C1. C4, C7, and C10, without 6 His-tagged crdR. D1. D4, D7, and D10 are 10 μL of 0.5 μM crdP 98, crdP53, crdP13, and crdP1 only respectively, D2, D5, D8, and D11 are same of .D1. D4, D7, and D10 mixed with 10 μL 0.5 mg/mL 6 His-tagged crdR protein; D3, D8, D9, and D12 are same of .D1, D4, D7, and D10 mixed with 10 μL 0.5 mg/mL BSA respectively, E1, E4, E7, and E10 are crdRP, celAP, and crdSP, E2, E5, E8, and E11 are same of E1, E4, E7, and E10 mixed with 10 μL of 0.25 mg/mL 6 His-tagged crdR protein respectively, E3, E6, E9, and E12 are same of E1, E4, E7, and E10 mixed with 10 μL of 0.5 mg/mL BSA respectively.