Table 1 Plasmids used for P. xanthii electroporation and their main features
From: Transient transformation of Podosphaera xanthii by electroporation of conidia
Plasmid designation | Size (bp) | Resistance and/or reporter genes | Reference |
|---|---|---|---|
pAN7-1 | 6756 | E. coli hygromycin B phosphotransferase gene (hph), under the control of A. nidulans gpd (glyceraldehyde-3-phosphate dehydrogenase) promoter | [33] |
pGPDGFP | 6400 | Green fluorescent protein gene (gfp) fused to the gpd promoter and the tryptophan synthetase (trpC) terminator from A. nidulans | [34] |
pIGPAPA | 5910 | E. coli hph under the control of A. nidulans trpC promoter/gfp under the control of IsL (isocitrate lyase) promoter from N. crassa | [35] |
pCPXHY1eGFP | 9100 | E.coli hph under the control of A. nidulans trpC promoter/Enhanced green fluorescent protein gene (egfp) under the control of A. nidulans gpd promoter | [32] |
pCPXBteGFP | 11630 | Carbendazim resistant β-tubulin gene (TUB2) from P. xanthii under the control of its own promoter/egfp under the control of A. nidulans gpd promoter | This study |