Figure 6
From: Transient transformation of Podosphaera xanthii by electroporation of conidia
Transformation of P. xanthii conidia with the plasmid pCPXBteGFP. (A) Untransformed colonies growing normally on non-treated cotyledons. (B) Growth of P. xanthii transformants resistant to carbendazim. Conversely to hygromycin B, almost no appreciable tissue damage was observed on zucchini cotyledons. (C-D) EGFP analysis of transformants by CLSM analysis. (C) No fluorescence signal was observed in untransformed colonies. (D) In resistant transformants the EGFP fluorescence signal was observed within the peripheral hyphae; however, no fluorescence was observed in conidia or subjacent hyphae. No colonies were observed on cotyledons treated with carbendazim and inoculated with untransformed conidia. Pictures were taken 15 d after electroporation of conidia. Scale bars 100 μm.