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Figure 2 | BMC Microbiology

Figure 2

From: Chlamydia pneumoniae effector chlamydial outer protein N sequesters fructose bisphosphate aldolase A, providing a benefit to bacterial growth

Figure 2

Pull-down assay with MS analysis and Western blotting showing that C. pneumoniae CopN interacts with human aldolase A. (A) MS data with nano-LC-MS/MS. Bold red amino acid sequences, peptide matched sequences. (B) Representative Western blotting pattern with a specific aldolase A antibody for elution obtained from pull-down assay with GST-fused chlamydial CopNs and HEp-2 cell lysates. Input, HEp- 2 cell lysates. GST, GST alone. Cpn, GST-fused C. pneumoniae CopN (GST-CpCopN). CtD, GST-fused C. trachomatis CopN (GST-CtCopN). Red arrow, aldolase A. Asterisk, fragmented proteins.

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BMC Microbiology

ISSN: 1471-2180

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