Figure 4

Ser/Thr-rich regions and hyper- O -glycosylated regions among proteins in the glyco-secretome. A: Percentages of proteins displaying regions with the indicated minimal Ser/Thr content (horizontal axis), both among the experimental glycoprotein set (red bars) and among all predicted secretome proteins (blue bars). B: Percentage of proteins with predicted hyper-O-glycosylated regions in the same two proteins sets, also analyzed for the indicated minimal abundances of predicted O-glycosylation sites (horizontal axis). C: Frequency distribution of Ser/Thr-rich and hyper-O-glycosylated regions along proteins for the experimental glycoprotein set. The total number of regions found in every possible 10% interval along protein length is represented. D: Relative abundance of proteins in the Δbcpmt mutants, as compared with the wild-type strain B05.10, estimated from the spectral counts for two protein sets: those displaying hyper-O-glycosylated regions (purple bars) and those without them (green bars). Asterisks indicate a significant difference for the two sets (t-test) at either 0.95 (*) or 0.99 (**) confidence level.