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Table 2 Differentially expressed miRNAs identified to be commonly presented in ma81-infected mouse lungs at both 1 dpi and 3 dpi

From: Differential microRNA expression following infection with a mouse-adapted, highly virulent avian H5N2 virus

miRNA

ma81‡

1dpi

3dpi

Fold change*

P value§

Fold change

P value

miR-100-5p†

2

0.035

2

0.007

miR-130a-5p†

4.5

0.001

2.2

0.03

miR-139-5p

4

0.031

3.8

0.003

miR-146b-3p†

3.4

0.011

4.1

0.046

miR-147-3p†

7.8

0.045

25.2

0.04

miR-151-5p†

7.3

0.027

2.2

0.002

miR-155-3p†

6.2

0.024

6.2

0.018

miR-223-3p†

7.1

0.005

8.9

0.013

miR-27a-5p

2.2

0.035

2.9

0.023

miR-29b-1-5p

2.7

0.043

2.2

0.021

miR-301a-3p†

−2.8

0.042

−2

0.049

miR-449a-5p

−2.5

0.046

−2.7

0.016

miR-495-3p†

2.4

0.039

2.3

0.004

miR-877-3p

3.1

0.04

4.4

0.04

  1. ‡ global cellular miRNA expression patterns in mouse lungs infected with ma81 were compared with those of uninfected control mouse lungs. Differentially expressed miRNAs with a p < 0.05 and fold change>2 are described.
  2. *Sequence reads were normalized to determine the number of transcripts per million (TPM) using the following formula: Normalized expression = Actual miRNA count/Total count of clean reads*1000000. Then, fold changes of miRNAs were evaluated using the following formula: Fold change = log2 (treatment/control).
  3. §P-value was calculated from the normalized expression values as previously described [66].
  4. †Significantly upregulated in both lungs infected with either wild type w81 or the mouse-adapted ma81 strain at all time points.