miRNA | ma81‡ |
---|
1dpi | 3dpi |
---|
Fold change* | P value§ | Fold change | P value |
---|
miR-100-5p†| 2 | 0.035 | 2 | 0.007 |
miR-130a-5p†| 4.5 | 0.001 | 2.2 | 0.03 |
miR-139-5p | 4 | 0.031 | 3.8 | 0.003 |
miR-146b-3p†| 3.4 | 0.011 | 4.1 | 0.046 |
miR-147-3p†| 7.8 | 0.045 | 25.2 | 0.04 |
miR-151-5p†| 7.3 | 0.027 | 2.2 | 0.002 |
miR-155-3p†| 6.2 | 0.024 | 6.2 | 0.018 |
miR-223-3p†| 7.1 | 0.005 | 8.9 | 0.013 |
miR-27a-5p | 2.2 | 0.035 | 2.9 | 0.023 |
miR-29b-1-5p | 2.7 | 0.043 | 2.2 | 0.021 |
miR-301a-3p†| −2.8 | 0.042 | −2 | 0.049 |
miR-449a-5p | −2.5 | 0.046 | −2.7 | 0.016 |
miR-495-3p†| 2.4 | 0.039 | 2.3 | 0.004 |
miR-877-3p | 3.1 | 0.04 | 4.4 | 0.04 |
- ‡ global cellular miRNA expression patterns in mouse lungs infected with ma81 were compared with those of uninfected control mouse lungs. Differentially expressed miRNAs with a p < 0.05 and fold change>2 are described.
- *Sequence reads were normalized to determine the number of transcripts per million (TPM) using the following formula: Normalized expression = Actual miRNA count/Total count of clean reads*1000000. Then, fold changes of miRNAs were evaluated using the following formula: Fold change = log2 (treatment/control).
- §P-value was calculated from the normalized expression values as previously described [66].
- †Significantly upregulated in both lungs infected with either wild type w81 or the mouse-adapted ma81 strain at all time points.