BMC Microbiology

Table 2 The numbers of bacteria (CFU) in different organs from animals

From: Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice

Salmonella strains		Colonization (i.p.)		Colonization (i.g.)
		log CFU per organ		log CFU per organ
		Liver	Spleen	Spleen	Cecum
(A) BALB/c mice	ST14028s	8.5 ± 0.5	7.7 ± 0.5	7.3 ± 0.5	7.5 ± 0.3
	T-prgJ	8.6 ± 0.5	7.9 ± 0.6	7.1 ± 0.5	7.5 ± 0.7
	T-sipA	9.4 ± 0.5	8.4 ± 0.7	7.4 ± 0.5	7.5 ± 0.7
	T-sipB	8.4 ± 0.5	7.5 ± 0.5	7.3 ± 0.5	7.7 ± 0.3
	T-sopE2	8.8 ± 0.5	8.3 ± 0.7	7.5 ± 0.5	7.4 ± 0.7
	T-spaO	8.5 ± 0.5	7.6 ± 0.8	7.0 ± 0.5	6.9 ± 0.6
	T-sptP	8.5 ± 0.5	7.6 ± 0.5	7.0 ± 0.5	6.9 ± 0.6
(B) SCID mice	ST14028s	8.7 ± 0.5	7.7 ± 0.5	7.9 ± 0.5	8.2 ± 0.6
	T-prgJ	8.9 ± 0.5	7.6 ± 0.7	7.3 ± 0.7	8.4 ± 0.6
	T-sipA	8.6 ± 0.5	7.5 ± 0.6	7.8 ± 0.6	8.9 ± 0.7
	T-sipB	8.9 ± 0.5	8.3 ± 0.5	7.6 ± 0.5	8.8 ± 0.7
	T-sopE2	8.9 ± 0.5	8.3 ± 0.6	7.6 ± 0.7	8.8 ± 0.4
	T-spaO	8.5 ± 0.5	8.0 ± 0.7	8.5 ± 0.7	8.6 ± 0.5
	T-sptP	8.9 ± 0.5	8.3 ± 0.6	7.6 ± 0.5	8.8 ± 0.5

*Mice were either infected intraperitoneally (i.p.) or intragastrically (i.g.) with 1 × 10⁵ CFU for BALB/c mice or 1 × 10² CFU for SCID mice. A group of 5 mice was infected and the organs were harvested at 5 (for i.p. infection) or 7 days (for i.g. inoculation) post infection. Each sample was analyzed in triplicate and the analysis was repeated at least twice. The CFU of the sample was expressed as the average of the values obtained. The concentrations of bacteria were recorded as CFU/ml of organ homogenate. The limit of bacteria detection in the organ homogenates was 10 CFU/ml.

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