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Table 2 DNA fragmentation levels obtained in strains of E. coli with different susceptibilities to CIP.

From: Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay

   

CIP dose

Strain

Mutations

MIC

MIC 1×

MIC 10×

MIC 100×

C-20

-

0.007

1.5 ± 0.3

6.7 ± 0.8

10.3 ± 2.5

C-15

Ser83Leu from GyrA

0.25

1.7 ± 0.3

6.2 ± 0.7

8.7 ± 1.1

1273

Ser83Leu and Asp87Tyr from GyrA

8

0

1.8 ± 0.3

2.7 ± 0.4

1383

Ser83Leu and Asp87Tyr from GyrA and Ser80Ile and Glu84Lys from ParC

128

0

0

0

J53

-

0.007

1.8 ± 0.8

9.2 ± 1.2

10.4 ± 2.0

J53qnrA1

Plasmid gene J53qnrA1

0.25

1.9 ± 0.4

9.5 ± 1.3

9.8 ± 0.9

  1. The level of fragmentation obtained by different CIP doses is indicated by the width of the halo of dispersion of DNA fragments and is measured in μm (mean ± standard deviation). MIC is in μg/ml.