PCR Primers, Probes and Targets | Sequence | Length | Fluorophore/Quencher | Tm Probe-target/Stem |
---|---|---|---|---|
RecF | 5' GTG GAT CTA TTG TAT TAG ATG AGG CTC TCG 3' | 30 | - | - |
RecR | 5' GCC AAA GTT CTG CAA CAT TAA CAC CTA AAG 3' | 30 | - | - |
NidoF | 5' CCA GCC ACA GAA TAC CAT CC 3' | 20 | - | - |
NidoR | 5' GGA CAT ACT CTG CTG CCA TC 3' | 20 | - | - |
Nidogen | 5' CGG CGC ACC CAG CTT CGG CTC AGT AGC GCC G 3' | 31 | TET/BHQ1 | 77°C/84°C |
Nidogen Target | 5' ta GGC GCT ACT GAG CCG AAG CTG GGT G at 3' | 29 | - | - |
RecA1 | 5' CCC GCG CGT CTG GCA AGA CTA CTT TAA CTC TTC GCG GG 3' | 38 | FAM/BHQ1 | 68°C/71°C |
RecA1 Target | 5' ta GAA GAG TTA AAG TAG TCT TGC CAG ACG at 3' | 31 | - | - |
RecA2 | 5' CGCGAG TCG TCT GGC AAG ACT ACT TTA A CTCGCG 3' | 34 | FAM/DABCYL | 73°C/67°C |
RecA2 Target | 5' ttG AGT TAA AGT AGT CTT GCC AGA CGA CTC tt 3' | 32 | - | - |
RecA3 | 5' CTG GCG GAT ATC CTA GGG GG CGC CAG 3' | 26 | FAM/BHQ1 | 75°C/75°C |
RecA3 Target | 5' ttG CGC CCC CTA GGA TAT CCG CCt t 3' | 25 | - | - |