Table 4 The PCR primers for PCR and size of PCR products
Primer | Target | DNA sequence (5' to 3') | Product Sizesize | Note |
|---|---|---|---|---|
Tem-F | bla TEM | GAAGATCAGTTGGGTGCACGAGT | 550 bp | This study |
Tem-R | Â | CAACTTTATCCGCCTCCATCCAGT | Â | Â |
STR-F1 | aadA2 | AGACGCTCCGCGCTATAGAAGT | 203 bp | (46) |
STR-R1 | Â | CGGACCTACCAAGGCAACGCT | Â | Â |
CS-F | CS region | GGCATCCAAGCAGCAAG | Variable | (47) |
CS-R | Â | AAGCAGACTTGACCTGA | Â | Â |
1.9CS-F | Flanking region of CS region | CTGCTGCGTAACATCGTTGCT | Variable | This study |
1.9CS-R | Â | GGCGAGATCATCAAGTCAGT | Â | Â |
ColE1-F | ColE1 ori T | CAAATGCTGTCCTTCCAGTGT | 225 bp | This study |
ColE1-R | Â | CTCAGTTCGGTGTAGGTCGT | Â | Â |
F-F | IncFI ori T | CAACAACGCGCCGACACCGT | 288 bp | This study |
F-R | Â | CCCTTCCTGTCGACGCTTCT | Â | Â |
R100-F | IncF2 ori T | CCACCAAAAGCACCACACACT | 266 bp | This study |
R100-R | Â | AGACACTCCTAGCAGCGCCT | Â | Â |
pSC138-F | IncI ori T | TGTCACGAACATCTGCCAGT | 193 bp | This study |
pSC138-R | Â | GAGAGAAAGTGCCCATGGCT | Â | Â |
IS26in-F | IS 26 | GGCACTGTTGCAAAGTTAGC | 820 bp | DQ390455.1 |
IS26in-R | Â | GGCACTGTTGCAAATAGTCG | Â | Â |
IS26out-F | Variable | GCTAACTTTGCAACAGTGCC | Variable | DQ390455.1 |
IS26out-R | Â | CGACTATTTGCAACAGTGCC | Â | Â |
Tn-F | Tn | ACCTAGATTCTACGTCAGTAC | Variable | (35) |
AmpC-F | AmpC | CAAGTTTGATTCCTTGGACTCT | Â | AY253913 |
AmpC-R | Â | CTCATCGTCAGTTATTGCAGCT | Â | Â |
SugE-R | sugE | GCCTGATATGTCCTGGATCGT | Â | Â |