Primer | Sequence (5' - 3') | Target | Accession no. | Region amplified | Amplicon length (bp) | PCR conditions (°C, s)* | ||
---|---|---|---|---|---|---|---|---|
 |  |  |  |  |  | Den | Ann | Ext |
U1 U2 | GCAGCCGTTTGGTCACGG CTATGCCACGCATCCCGACC | ureA-ureC | DQ350880 AM286415 Z18865 | 275...2896 1075847...1078426 325...2907 | 2622 2580 2582 | 94, 60 | 62.0, 110 | 72, 110 |
ureA1 ureA2 | GGAGGGCTTATGCAGCTCACCCCAAG TTGCCATCTCTGGCCCCTTCCA | ureA | DQ350880 AM286415 Z18865 | 1...161 1075573...1075733 51...211 | 161 161 161 | 94, 60 | 61.4, 60 | 72, 60 |
ureAB1 ureAB2 | CAATGGAAGGGGCCAGAGATGG GTAAGCCGCAGCACGGTCAAACTC | ureA-ureB | DQ350880 AM286415 Z18865 | 137...579 1075709...1076210 187...688 | 443 502 502 | 94, 60 | 60.3, 60 | 72, 60 |
ureAB3 ureAB4 | GCAGCTCACCCCAAGAGAAGTTGA AATTTGAGGCATCTGTCGCTCCTT | ureA-ureB | DQ350880 AM286415 Z18865 | 12...1015 1075584...1076608 62...1086 | 1004 1025 1025 | 95, 60 | 56.9, 110 | 72, 60 |
ureB1 ureB2 | ATTGCAGAGGATTAAAGCATGAGC AGCGGAACTTCGGTTTCATCACC | ureB | DQ350880 AM286415 Z18865 | 349...650 1075920...1076281 398...759 | 302 362 362 | 94, 60 | 60.0, 60 | 72, 60 |
ureBC1 ureBC2 | TGCGGCTTACGGAAAAAGGCTGAATA GCCGAGAAATTTGAGGCATCTGTCG | ureB-ureC | DQ350880 AM286415 Z18865 | 570...1022 1076201...1076615 679...1093 | 453 415 415 | 94, 60 | 60.3, 60 | 72, 60 |
ureC1 ureC2 | AAAGGAGCGACAGATGCCTCAAA GAAACCTGAATATCCATTTCATCCGCCAT | ureC | DQ350880 AM286415 Z18865 | 991...1749 1076584...1077342 1062...1823 | 759 759 762 | 94, 60 | 63.2, 60 | 72, 60 |
ureC3 ureC4 | GGCTATAAAGTTCACGAAGACTG CAAAGAAATAGCGCTGGTTCA | ureC | DQ350880 AM286415 Z18865 | 1661...2717 1077254...1078310 1735...2791 | 1057 1057 1057 | 94, 60 | 52.9, 60 | 72, 60 |
ureC1 ureC4 | AAAGGAGCGACAGATGCCTCAAA CAAAGAAATAGCGCTGGTTCA | ureC | DQ350880 AM286415 Z18865 | 991...2717 1076584...1078310 1062...2791 | 1727 1727 1730 | 94, 60 | 50.0, 60 | 72, 120 |
ureCE1 ureCE2 | GCGCTGGATGACGGTGTGAAAGAG ATGTAAGCCGGAGCCATGAGGTTC | ureC-ureE, ureE | DQ350880 AM286415 | 2504...3552 1078097...1079082 | 1019 986 | 94, 60 | 61.0, 60 | 72, 60 |
ureE1 ureE2 | ACCATGATGGATTCCGTGATGAGA GTGAAGGCCCCGACCGGCAGTACG | ureE-ureF | DQ350880 AM286415 | 3364...3734 1078894...1079270 | 371 377 | 95, 60 | 58.7, 60 | 72, 60 |
ureF1 ureF2 | TGAATGCATCAGATCTGATTCGTA ACATCCACAATAGGGACATAAGA | ureF | DQ350880 AM286415 | 3668...4304 1079204...1079840 | 637 637 | 95, 60 | 50.0, 60 | 72, 60 |
ureFG1 ureFG2 | CAATATGGCGTGGCGATGACAAT CCACCGGGCCACCAATACCAA | ureF-ureG | DQ350880 AM286415 | 4132...4535 1079668...1080070 | 403 401 | 95, 60 | 55.7, 60 | 72, 60 |
ureG1 ureG2 | GAATAGCCATTCAACCGATAAAC CGCATAATCATATCCACCAAC | ureG | DQ350880 AM286415 | 4474...5091 1080009...1080626 | 618 618 | 95, 60 | 51.3, 60 | 72, 60 |
ureG1 ureD2 | GAATAGCCATTCAACCGATAAAC TTCCGGCAATGTCACACCGAGAAT | ureG-ureD, ureD | DQ350880 AM286415 | 4474...6099 1080009...1081634 | 1626 1626 | 95, 60 | 50.4, 60 | 72, 120 |
ureD1 ureD2 | AGCCAGAATATCGTGGAAACTCCT TTCCGGCAATGTCACACCGAGAAT | ureD | DQ350880 AM286415 | 5146...6099 1080681...1081634 | 954 954 | 95, 60 | 50.0, 60 | 72, 60 |
ureD3 ureD4 | TTGTTAACCCCCAAAGAGCATCAT CTGCCGGATTCCCTTCGCCATAG | ureD-yut | DQ350880 AM286415 | 5884...6416 1081419...1081950 | 533 532 | 95, 60 | 58.0, 60 | 72, 60 |
Yut1 Yut2 | CGCGGCTGTGCTCAAGTC GTGCTGGCATCACATCTTTATTAGG | yut | AM286415 | 1081851...1082745 | 895 | 95, 60 | 50.0, 60 | 72, 60 |