Helicobacter pylori lipopolysaccharide modification, Lewis antigen expression, and gastric colonization are cholesterol-dependent

Table 1 Primer sequences.

primers for allelic disruption ^a
CAT fwd [41]	GATATAGATTGAAAAGTGGAT	F5^b
CAT rev [41]	TTATCAGTGCGACAAACTGGG
cgtfwd	atggttattgttttagtcgtgga
cgtM3	ATCCACTTTTCAATCTATATCatatggtggatatagcggtaatg
cgtM5	CCCAGTTTGTCGCACTGATAAttaaaaacttgcaccctttatgt
cgtrev	ctctgatcgcttcttcataaact
pmifwd	atgaaaattaaaaatatcttactgagtggg
pmiM3	ATCCACTTTTCAATCTATATCatctaaaccattagggctttcaatatac
pmiM5	CCCAGTTTGTCGCACTGATAActttagtgaacgaggtagaaacaaac
pmirev	ttttgtctgttaaaatcatcatcaat
lpxE fwd	atgaaaaaattcttatttaaacaaaaattttgtgaaagc
lpxEM3	ATCCACTTTTCAATCTATATCcccaaacgctgatcgttgat
lpxEM5	CCCAGTTTGTCGCACTGATAAcgagcgcccttatggag
lpxErev	ttaaggctttttggggcttgtaaa
eptAfwd	ttggcatcattattccatctgaggt
eptAM3	ATCCACTTTTCAATCTATATCgcaacaccccaaaaacaacgata
eptAM5	CCCAGTTTGTCGCACTGATAAagcctgattaacgcctatgaca
eptArev	ttactcttttttgtgtttaagcagatctaaagaa
*additional primers for confirmation of gene disruption*
G27_951fwd	agtgattcaagatggcgtgaaaa	F1
G27_953rev	ccaagctcaatcatttctttgtcttt	R1
G27_37fwd	cggcatggggatcaatcaag	F2
G27_39rev	ctcccgtcttgcccggtaac	R2
G2719fwd	gggcgataaaatcgtgtttca	F3
G2721rev	tcccctttatcgtttatgctaatga	R3
G2720fwd	cccaaactgagcgctaaca	F4
G2722rev	aagaaatttcaaggtataatagtttccaag	R4

^aRespective gene numbers in public databases for 26695 and G27 are as follows: [cgt: hp0421, G27_952], [pmi (rfbM): hp0043, G27_38], [lpxE: hp0021, G27_20], [eptA: hp0022, G27_21].
^bRighthand column lists the brief primer designations used in Figure 1.

ISSN: 1471-2180