Figure 3From: Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersoniiSplicing of hsp70 mRNA is inhibited in B. emersonii cells exposed to cadmium. Nuclease Sl protection assays were performed using the 5' end-labeled probe, depicted above the autoradiogram, and 50 μg of yeast tRNA in the presence or absence of nuclease S1 (lanes 1 and 2) as control, 50 μg of total RNA isolated from 60 min sporulating cells (lane 3), cells submitted to heat shock at 38°C and 42°C for 30 min (lanes 4 and 5, respectively), cells submitted to 250 μM CdCl2 for 30 min and 60 min (lanes 6 and 7, respectively), cells submitted to 500 μM H2O2 for 30 min and 60 min (lanes 8 and 9, respectively).Back to article page