Table 2 Proteins encoded by IVIAT selected clones

Cell envelope

structure

ysirk1

YSIRK Gram-positive signal peptide

cell wall

EF127690

ysirk2

YSIRK Gram-positive signal peptide

cell wall

EF127720

ss-1616

hypothetical protein SsuiDRAFT 0718

cell wall

EF127691

cwh

Cell wall hydrolase/autolysin, peptidoglycan catabolism

cell wall

EF127695

trag

TRAG protein, essential for DNA transfer in bacterial conjugation

Membrane

EF127701

srt

sortase-like protein/Sortase family

Cytoplasmic

EF127708

nlpa

NLPA lipoprotein

Cytoplasmic

EF127718

Regulation

lac

LacI:Periplasmic binding protein/LacI transcriptional Regulator

EF127711

hprk

HPr(Ser) kinase, two-component signal transduction system/two-component sensor activity/regulates carbohydrate

Cytoplasmic

EF127733

flps

FlpS, regulation in arginine deiminase system

Cytoplasmic

EF127749

Molecule synthesis

ss-896

Tagatose-6-phosphate kinase/pfkB family carbohydrate kinase

Cytoplasmic

EF127693

ss-1611

Aspartate kinase/Amino acid kinase family

Cytoplasmic

EF127726

ss-1759

Amino acid biosynthesis/glutamate-cysteine ligase activity

Cytoplasmic

pol1

DNA polymerase I

Cytoplasmic

EF127735

cpn60

60 kDa chaperonin (Protein Cpn60) (groEL protein)/TCP-1/cpn60 chaperonin family

Cytoplasmic

EF127737

prpn

Primosomal protein n

Cytoplasmic

EF127740

pol3

DNA polymerase III, epsilon subunit:DNA polymerase III, alpha subunit, Gram-positive type

Cytoplasmic

EF127741

EF127751

Substance and energy metabolism

gh3

Glycoside hydrolase, family 3, N-terminal/Tim barrel glycosyl hydrolase superfamily. carbohydrate metabolism/hydrolase activity, hydrolyzing O-glycosyl compounds

Cytoplasmic

EF127713

ss-999

hypothetical protein, carbohydrate metabolism/hydrolase activity

Cytoplasmic

EF127746

ss-1647

tRNA (guanine-N(7)-)-methyltransferase/Methyltransferase superfamily, Central intermediary metabolism

unknown

EF127722

ss-862

Uracil DNA glycosylase/Uracil DNA glycosylase superfamily

Cytoplasmic

EF127729

ss-802

ATPase

Cytoplasmic Membrane

EF127747

ss-1766

protein metabolism/ATP-dependent Clp protease, ATP-binding subunit

Cytoplasmic

EF127719

ss-607

ABC transporter

Cytoplasmic Membrane

EF127697

sdh

L-serine dehydratase, alpha subunit

Cytoplasmic Membrane

EF127704

ss-1617

Fructokinase/Actin-like ATPase Superfamily

unknown

EF127707

ppc

Phosphoenolpyruvate carboxylase

EF127727

ss-1305

ABC transporter

Cytoplasmic Membrane

EF127736

Transport

ss-1955

conserved hypothetical protein, transport and binding

unknown

EF127692

ss-273

ABC transporter, transport and binding/coupled to transmembrane movement of substances

Cytoplasmic Membrane

EF127694

ss-1298

ABC transporter

Cytoplasmic Membrane

EF127714

ss-1829

peptidase, S54 (rhomboid) family protein/Rhomboid family

Cytoplasmic Membrane

EF127742

ss-403

PTS system sorbose subfamily IIB component

cytoplasmic

EF127754

Translation

exc-b

Excinuclease ABC, B subunit

Cytoplasmic

EF127698

EF127700

ss-887

O-acetylhomoserine sulfhydrylase/PLP dependent aminotransferase superfamily

Cytoplasmic

EF127706

ss-349

possible product: hypothetical protein SMU_684

Cytoplasmic

EF127738

ss-1935

Ribosomal protein S4, bacterial and organelle form

Cytoplasmic

EF127739

Others

ss-154

3-phosphoglycerate kinase, phosphoglycerate kinase activity/glycolysis

Cytoplasmic

EF127702

ss-485

Metal-dependent phosphohydrolase, HD subdomain/HD/PDEase superfamily

Cytoplasmic

EF127730

ss-1478

Inorganic diphosphatase,

Cytoplasmic

EF127734

smc

SMC protein, N-terminal: Structural maintenance of chromosome protein SMC, C-terminal: SMCs flexible hinge

Cytoplasmic

EF127743

Function unknown

g19

unknown

EF127696

g59

Protein of unknown function DUF150

Cytoplasmic

EF127717

g77

unknown

EF127724

g78

unknown

EF127725

g98

hypothetical protein Franean1DRAFT_0529

EF127732

g130

unknown

EF127744

g132

unknown

EF127745