Table 1 Bacterial strains and plasmids used in this study
Strains or plasmids | Serotype, Genotype and/or phenotype | Reference/source |
|---|---|---|
Strains | Â | Â |
HA9801 | serotype 2;cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+ | Jiangsu outbreak SS2 isolate, 1998, China |
ZY05719 | serotype 2; cps2J+, mrp+, ef+, sly+, gapdh+, gdh+, orf2+ | Sichuan outbreak SS2 isolate, 2005, China |
T15 | serotype 2; cps2J+, mrp-, ef- | The Netherlands |
Plasmids | Â | Â |
pET30(abc) | Expression vectors allowing cloning of fragments in each of three reading frames; Kanr | Novagen |
pMRP | pET30(a) with partial mrp gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr | This work |
pEF | pET30(a) with partial ef gene amplified from strain ZY05719, and cloned into EcoRI and XhoI sites, in vector, Kanr | This work |
pGAPDH | pET32(a) with partial gapdh gene amplified from strain ZY05719, and cloned into BamHI and SalI sites, in vector, Ampr | Previous work |