Figure 3
From: Cytokinesis is blocked in mammalian cells transfected with Chlamydia trachomatis gene CT223
Cytosolic production of CT223p and CT223/179p from C. trachomatis serovar D/UW3 leads to a multinuclear phenotype within mammalian cells. The vector pcDNA4/HisMaxC was used in each construct. Full length CT223p (panel A) and CT223/179p (panel B) were produced within cells following transfection of pcDNA4-based plasmids. Each was detected with anti-6 × His monoclonal antibodies (red). Microtubules were detected by labeling with specific anti-tubulin antibodies (green). The nuclei are labeled with DAPI (blue). Panel A; McCoy cell transfected with pcDNA4/HisMaxC encoding CT223p. Three nuclei are localized inside of a single cell expressing CT223. Panel B; McCoy cells transfected with pcDNA4/HisMaxC encoding carboxy-terminal CT223/179p. The scale bar in B indicates 10 microns for each panel.