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Table 1 Biological material used in this work.

From: Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci

Species

Isolate: reference

Origin (year, location)

Issue addressed

A. astaci type 1

L1

Astacus astacus

(1962, Sweden)

CHI, MCA, TaqMan

A. astaci type 1

Ra

A. astacus

(1973, Sweden)

CHI

A. astaci type 1

Sv

A. astacus

(1970, Sweden)

CHI, MCA, TaqMan

A. astaci type 2

Hö

A. astacus

(1974, Sweden)

CHI, Chi activity, Western, PCR

A. astaci type 2

Ti

A. astacus

(1970, Sweden)

CHI

A. astaci type 2

Yx

A. astacus

(1973, Sweden)

CHI

A. astaci type 3

Kv1

Pacifastacus leniusculus

(1978, Sweden)

CHI

A. astaci type 4

Pc

Procambarus clarkii

(1992, Sweden)

CHI

A. astaci

GB04

(CBS 121.537)

P. leniusculus

(2004, Ganaubach, Austria)

CHI, PHYLO, RACE, GX, MCA, TaqMan

A. astaci

GKS07

(CBS 121.538)

A. astacus

(2007, Gleinkersee, Austria)

PHYLO

A. astaci

Z12

(CBS 117.160)

P. leniusculus

(2004, Zöbernbach, Austria)

PHYLO

A. frigidophilus

NJM 9665

(ATCC 204464): [6, 61]

egg of Onchorhynchus masou (1996, Japan)

CHI, MCA, TaqMan

A. frigidophilus

SAP472: [30]

Austropotamobius pallipes (2008, Spain)

CHI

A. invadans

WIC: [6]

Brevoortia tyrannus

(2004, USA)

CHI, MCA, TaqMan

A. laevis

CBS 107.52

unknown

(1952, unknown)

CHI, MCA, TaqMan

A. helicoides

CBS 210.82

unknown

(1982, former USSR)

CHI, MCA, TaqMan

A. repetans

LK29

P. leniusculus

(2004, Leithakanal, Austria)

CHI, PHYLO

A. irregularis

CBS 278.81

pond

(1981, The Netherlands)

CHI, MCA, TaqMan

Achlya racemosa

CBS 578.67

unknown

(1967, Great Britain)

CHI

Leptolegnia caudata

CBS 680.69

unknown

(1969, Canada)

CHI, MCA, TaqMan

Saprolegnia parasitica

CBS 540.67

fish hatchery

(1967, Great Britian)

CHI

Aspergillus sp.

not assigned

horse food

(2004, Vienna, Austria)

MCA

Fusarium solani

CBS 181.29

unknown

(1929, Germany)

CHI

Trichosporon cutaneum

DSM 70675

sulfite liquor waste

CHI

  1. Western: western-blot analysis, CHI: partial sequencing of homologous chitinase gene(s), RACE: rapid amplification of cDNA ends, PHYLO: determination of ITS nrDNA sequences for phylogenetic analysis, GX: temporal gene expression of Chi2 and Chi3, MCA: qPCR/MCA for qualitative detection of A. astaci, TaqMan: TaqMan qPCR