Figure 3

Klebsiella induced cytotoxicity is observed by disintegration of host genomic DNA and loss of host plasma membrane integrity. A. Ethidium bromide staining after agarose gel-electrophoresis of genomic DNA isolated from A549 epithelial cells infected with K. pneumoniae strains 52145, 43816, 1850 or 52K10. B. A549 lung epithelial cells were not infected (left), infected with K. pneumoniae strain 52K10 (middle), or strain 52145 (right). The cells were stained with ethidium bromide and analysed by fluorescence microscopy. Necrotic or apoptotic cells had normal/condensed nuclei that were brightly stained with ethidium bromide and appeared red (white arrows).