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Table 2 Stability of luminescent bacteria evaluated as emitting concentration and luminescence detection.

From: Photonic plasmid stability of transformed Salmonella Typhimurium: A comparison of three unique plasmids

 

Luminescent Salmonella typhimurium

 

Day of Culture (Emitting Concentration; CFU)

Plasmid Type

0

6

10

   pAK1-lux

1.2 × 108 ± 7.2 ×106a,x

7.4 × 107 ± 1.1 × 107b,x

4.2 × 107 ± 7.2 × 106c,x

   pXEN-1

9.7 × 107 ± 7.2 × 106a,x

7.0 × 107 ± 7.8 × 106b,x

4.4 × 107 ± 7.2 × 106c,x

   pCGLS-1

1.2 × 108 ± 7.2 × 106a,x

4.6 × 107 ± 1.1 × 107b,y

1.3 × 107 ± 7.2 × 106c,y

 

Luminescent Salmonella typhimurium

 

Day of Culture (Photonic Detection; RLU/s)

Plasmid Type

0

6

10

   pAK1-lux

7811 ± 159a,x

5550 ± 159b,x

3839 ± 158c,x

   pXEN-1

7149 ± 159a,y

4898 ± 171b,y

3552 ± 159c,y

   pCGLS-1

4753 ± 159a,z

1921 ± 242b,z

708 ± 159c,z

  1. Superscripts differ within plasmid a,b,c (P < 0.05).
  2. Superscripts differ between plasmids x,y,z (P < 0.05).
  3. Stability of luminescent Salmonella typhimurium with three different plasmids (pAK1-lux, pXEN-1, and pCGLS-1) in 96-well format (100 μl/well); Percent emitting and non-emitting evaluations at day 0, 6, and 10 of in vitro culturing without ampicillin selection.