Figure 6

Cholesterol depletion affects total CD81 cell surface expression. A, Flow cytometry analysis of CD81 and CD151 expression on the cell surface of Huh-7w7/mCD81 cells. Upper panels: cells were treated with 7.5 mM of MβCD (MβCD) or left untreated (NT). Middle panels: cells were treated with 7.5 mM of MβCD (MβCD) followed by 2.5 mM of MβCD-Cholesterol (MβCD + Chol). Lower panels: cells were treated with 2.5 mM of MβCD-Cholesterol (Chol) or left untreated (NT). B, Cells were treated with 7.5 mM of MβCD (MβCD) followed by increasing concentrations (in mM) of MβCD-Cholesterol (MβCD + Chol) and total cell surface CD81 expression compared to untreated cells (NT) was measured using MT81 mAb.