The mycobacterial DNA-binding protein 1 (MDP1) from Mycobacterium bovis BCG influences various growth characteristics

Table 1 Primers and probe used in this study.

Gene	Primer pair, probe	Sequences of primers and probes^a	References accession no.
MDP1	MDAS11 MDAS2	GGG AAGCTT CGGAGGGTTGGGATGAACAAAGCAGA GTG CTGCAG ACGCACAATCGTGTCAACGACATT	GenBank: AB013441
aph	Tn903/S1 Tn903/AS1	CGA GGC CGC GAT TAA ATT CCA AC TGA GTG ACG ACT GAA TCC GGT GAG A	GenBank: DQ115380
MDP1 antisense construct	MDAS1 pMV261FW	GAA TTC GGA GGG TTG GGA TGA ACA AAG CAG A GAG GAA TCA CTT CGC AAT GGC	This study
85B antigen	MY85FW MY85BW 85Bprobe	TCAGGGGATGGGGCCTAG GCTTGGGGATCTGCTGCGTA (FAM)-TCGAGTGACCCGGCATGGGAGCGT-(TAMRA)	[20] [21]

^aRestriction sites added to the primers for cloning purposes are italicised and underlined.

ISSN: 1471-2180