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Table 2 Proteins identified in the outer membrane fraction of cultures grown in M9 medium.

From: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Section

Protein

Acc.No.

MW(cal)

Size range

Coverage (ESI-MS/MS)

Score (ESI-MS/MS)

Coverage (MALDI)

Score (MALDI)

1

TonB-dependent outer membrane receptor with signalP

AAM 42139

100858

100–150

19

664

114

16

 

Oar putative outer membrane receptor protein, tonB-dependent with signalP

AAM41771

117723

 

10

295

64

12

 

TonB-dependent outer membrane receptor (C-terminal fragment) with signalP

AAM43353

110414

 

8

271

65

6

 

TonB-dependent outer membrane receptor (C-terminal fragment) with signalP

AAM42431

106797

 

7

141

-

-

 

TonB-dependent outer membrane receptor with signalP

AAM42675

103487

 

3

53

57

5

2

TonB-dependent outer membrane receptor with signalP

AAM42139

100858

80–100

14

630

124

18

 

TonB-dependent outer membrane receptor with signalP

AAM42044

83577

 

21

628

-

-

 

FpvA TonB-dependent ferripyoverdine receptor precursor with signalP

AAM42628

81083

 

20

506

116

16

 

PhuR outer membrane hemin receptor, tonB-dependent with signalP

AAM41930

85127

 

17

317

92

13

 

TonB-dependent outer membrane receptor with signalP

AAM42316

84374

 

8

218

64

8

 

Outer membrane protein with signalP

AAM40663

90600

 

6

113

72

8

 

Bgl exported beta-glucosidase with signalP

AAM41065

91830

 

4

34

-

-

3

Egl exported cellulose with signalP

AAM42791

52209

50–80

7

142

68

6

 

OmpA family outer membrane protein with signalP

AAM40245

39338

 

8

98

72

16

 

Ffh Signal recognition particle protein

AAM40492

49312

 

6

31

-

-

4

OmpA family outer membrane protein with signalP

AAM40245

39338

40–50

34

581

18

93

 

FadL outer membrane fatty acid porin with signalP

AAM39336

47455

 

11

229

-

-

 

TufA (elongation factor Tu)

AAM40191

43181

 

14

70

88

12

5

OmpA family outer membrane protein with signalP

AAM40245

39338

30–40

32

402

73

16

 

Putative secreted protein

AAM39862

29865

 

5

41

63

9

 

Egl exported cellulose

AAM42791

52209

 

8

39

55

6

 

XpsE Type II secretory pathway ATPase

AAM39976

64291

 

3

29

-

-

 

FliC Flagellin A (Flagellin core protein)

AAM41230

40380

 

3

29

64

8

6

OmpA family outer membrane protein with signalP

AAM40245

39338

27–30

29

515

92

18

 

Putative secreted protein

AAM39862

29865

 

3

40

56

8

 

FadL (outer membrane fatty acid porin)

AAM39336

47114

 

3

26

-

-

7

OmpA family outer membrane protein with signalP

AAM40245

39338

24–27

9

88

73

16

 

Putative exported protein

AAM40085

35080

 

8

60

63

12

 

UptE Outer membrane protein with signalP

AAM39911

31591

 

26

37

73

32

8

OmpW3 ompW family outer membrane protein

AAM39855

23494

20–24

7

55

58

13

 

XpsH General secretion pathway protein

AAM39979

18208

 

11

47

63

21

 

Putative secreted protein

AAM39862

29865

 

7

26

74

14

9

UptD outer membrane protein with signalP

AAM39910

17116

15–20

13

57

60

14

 

WxcE exported protein

AAM39921

20490

 

11

26

54

9

10

Putative exported protein with signalP

AAM40594

10647

10–15

15

117

-

-

 

UptD outer membrane protein with signalP

AAM39910

17116

 

6

33

64

16

  1. The section indicates the gel-section excised from the corresponding SDS-PAGE. The values indicated under MW(cal) gives the calculated molecular weight of the identified proteins. The size range relates the excised gel-section with regard to the molecular weight standard on the SDS-PAGE. Score (MOWSE-score) and coverage (sequence coverage) relate to the two approaches (ESI-MS/MS or MALDI-TOF-MS) utilized for the identification of the proteins on the SDS-PAGE.
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BMC Microbiology

ISSN: 1471-2180

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