Table 1 Strains and plasmids used for this study.
Strain | Genotype | Reference or source |
|---|---|---|
168 | trpC2 | Laboratory stock |
IFO3335 | Non-laboratory strain | Laboratory stock |
IA95 | trpC2 degU32 | BGSC |
OAM392 | trpC2 thrC ::swrAA N (Spr) | This study |
TT714 | trpC2 leuC7 degU (Kmr) | [46] |
TT719 | trpC2 leuC7 ΔdegS (in frame deletion) | [46] |
OAM369 | trpC2 ΔdegS (in frame deletion, yvyE ::Cmr::Emr) | This study |
OAM385 | trpC2 amyE ::flgB-lacZ 1D (Cmr) | This study |
OAM386 | trpC2 amyE ::flgB-lacZ 1D (Cmr) thrC ::swrAA N (Spr) | This study |
OAM387 | trpC2 amyE ::flgB-lacZ 1D (Cmr) degU (Kmr) | This study |
OAM388 | trpC2 amyE ::flgB-lacZ 1D (Cmr) ΔdegS (Emr) | This study |
OAM389 | trpC2 amyE ::flgB-lacZ 1D (Cmr) thrC ::swrAA N (Spr) degU (Kmr) | This study |
OAM390 | trpC2 amyE ::flgB-lacZ 1D (Cmr) thrC ::swrAA N (Spr) ΔdegS (Emr) | This study |
OAM391 | trpC2 amyE ::flgB-lacZ 1D (Cmr) degU32 | This study |
OAM371 | trpC2 amyE ::sacB-lacZ S-WT (Cmr) | This study |
OAM372 | trpC2 amyE ::sacB-lacZ S-WT (Cmr) degU (Kmr) | This study |
OAM373 | trpC2 amyE ::sacB-lacZ S-WT (Cmr) ΔdegS (Emr) | This study |
OAM393 | trpC2 amyE ::sacB-lacZ SC-IR2 (Cmr) | This study |
OAM394 | trpC2 amyE ::sacB-lacZ SC-IR2 (Cmr) degU (Kmr) | This study |
OAM395 | trpC2 amyE ::sacB-lacZ SC-IR2 (Cmr) ΔdegS (Emr) | This study |
Plasmid | Description | Reference or source |
pDG-swrAAN | pDG1731 carrying swrAA N | This study |
pCa-yvyE | pCA191 carrying a part of yvyE and a promoter of degSU | K. Kobayashi |
pCm::Emr | Ampicillin and erythromycin resistance | [48] |
pTYB-degU | pTYB2 carrying degU, ampicillin resistance | This study |
pET-degS | pET28b carrying degS, kanamycin resistance | K. Kobayashi |
pDG-His-degU | Plasmid carrying T5 promoter-His6-degU, ampicillin resistance | [19] |
pIS284 | Insertion vector to amyE, chloramphenicol resistance, lacZ | I. Smith |
pIS284-flgB | pIS284 carrying a promoter region of flgB | This study |
pIS284-sacB | pIS284 carrying a promoter region of sacB | This study |