Figure 2

Footprint analysis of the flgB promoter using His-DegU. A. Preparation of the probes and sequencing ladders was the same as that in Figure 1. For footprint assays, we used the procedures developed for biotinylated DNA probes as described previously [47]. The sequencing ladder is shown in lanes G, A, T and C. The brackets, dotted brackets and arrowheads indicate the protected regions, weakly protected regions and hypersensitive sites, respectively. 1. No protein. 2, 0.15 μM of His-DegU; 3, 0.3 μM of His-DegU. No His-tagged DegS was added. B. The nucleotide sequences of the promoter region are shown. Dotted arrows above the sequences indicate the putative DegU-binding motifs. The numbers indicate the nucleotide positions relative to the transcription start site.