Table 3 Primers used in this study
Primer | Sequence |
|---|---|
YchF-up5' | AAGTACACAAGAGAGTCGTCCGAT |
YchF-up3' | TTGGGCCCTCTCCGTTTTCATTTCAATCCC |
YchF-down5' | TTGGATCCAATGGTGTCAATTAGGTTGGAA |
YchF-down3' | TGATCATATTCTTCACCAACTGAGA |
YchF-C1 | GAAATTTCAGCAAGCACTCC |
YchF-C2 | ACTGATGGATAAACGCGTGT |
RluD-up5' | TTCTTGCCACCAACTATTACGGC |
RluD-up3' | TTTGGGCCCAGCCTTATCCAAACGCAGAC |
RluD-down5' | TTTGGATCCAATTTAAAGCAGATATCCCAGAGA |
RluD-down3' | ATGTTGAAATATTGACAAAACCGTCT |
RluD-C1 | TCAGTATGCCAATCCTGAAG |
RluD-C2 | CTTGATACCACCAGTTCCC |
DprA-up5' | TTGTCCTATCTTGTGATTGTGCTC |
DprA-up3' | TTTGGGCCCTTCTACTCATCTATCTATTCGT |
DprA-down5' | TTTGGATCCAGTGGGCAAGATGTTCTTGC |
DprA-down3' | CGATAGAGGCGATAAGCATGGC |
DprA-C1 | GAGTGAAATCGAAGCCCTC |
DprA-C2 | AATCACGGGAGTCGAATCC |
HemK-up5' | CAACTACAAGTTGTAGAAGACCGT |
HemK-up3' | TTTGGGCCCTGAGCTAATTTCATTATTTGTTTA |
HemK-down5' | TTTGGATCCTTGGTCAAGATAGGATGGTTGT |
HemK-down3' | TCTCCTTTATTGTGTGACTAGTCC |
HemK-C1 | GAGAATGAAGTAGTAGCAATC |
HemK-C2 | CAGAGATCAGCATCATATGC |
HemK-point1 | CAACTCTTATTAAAAATTTGAAAATAATTGAGC (point mutations in bold) |
HemK-point2 | TTTCAAATTTTTAATAAGAGTTGATAAGACAAGGAG (point mutations in bold) |
YebC-up5' | TTTGCCACTAGTGCGTAAGCGG |
YebC-up3' | TTGGGCCCTACGTCCCATTAGGAATCTCC |
YebC-down5' | TTGGATCCACCTATAGAACATGATCCTAAGTG |
YebC-down3' | CTTCCCAATAGCCCAGATAGCC |
YebC-C1 | TTAGAGAGTGTACCGGGC |
YebC-C2 | AGGACTGTTTTGGAAAGGC |
ErmApa | TTTTTGGGCCCTTCGTGTTCGTGCTGACTTGC |
ErmBam | TTTTTGGATCCGATGTTGCTGATTAAGACGAGC |
SP1022-point1 | TCTGTAGGCTAAACGACAGCTCAACCATTT (point mutations in bold) |
SP1022-point2 | AAATGGTTGAGCTGTCGTTTAGCCTACAGA (point mutations in bold) |
SP1022-up5' | TTTGCCACTAGTGCGTAAGCGG |
SP1022-up3' | TTGGGCCCTACGTCCCATTAGGAATCTCC |
SP1022-down5' | TTGGATCCACCTATAGAACATGATCCTAAGTG |
SP1022-down3' | CTTCCCAATAGCCCAGATAGCC |
SP1022-C1 | TCAGATTGCTCAAGATGAGC |
SP1022-C2 | TTCCACCTGAAATAATACGGA |
SP1023-point1 | GGTTGATGTCTCATATAGCTTACACATCAG (point mutations in bold) |
SP1023-point2 | CTGATGTGTAAGCTATATGAGACATCAACC (point mutations in bold) |
SP1023-up5' | AGATTGGTCAGTGACGGCA |
SP1023-up3' | TTGGGCCCAGTTTCTCTTAAATCTCTTAGC |
SP1023-down5' | TTGGATCCAGAAACGGTTTATTCGCATCT |
SP1023-down3' | TTCCACCTGAAATAATACGGA |
SP1023-C1 | TAGTGGAGCTATTGCTCTC |
SP1023-C2 | ATCCAACAAGTTTTGAGCAAC |