Figure 4

Immunofluorescent images of P. gingivalis in KB, EC and SMC 48 hours post-invasion (M.O.I. of 100). Micrographs A, B and C represent infected KB, EC and SMC, respectively. Micrographs D, E, and F represent infected KB, EC and SMC using the spin inoculation protocol. Scale bar is 50.0 μm. For images C and F, murine monoclonal anti-P. gingivalis antibody, 61BG1.3 was used followed by Cy3-labeled secondary antibodies. For smooth muscle actin (SMA), murine monoclonal anti-SMA antibody was used followed by FITC labeled secondary antibodies. Since both Cy3- and FITC-labeled secondary antibodies stained murine monoclonal antibodies, P. gingivalis may appear yellow. VWF, von Willebrand factor.