Evaluation of an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of Chlamydia trachomatis infections

Table 2 Primer sequences, size and location of DNA fragments in the omcB gene.

Vector	Region	Primer^a	Sequences^b	Size of DNA fragment (bp)	Location of DNA fragment (bp)^c
PinPoint Xa-1	Nt	F R	CCCTACGAACAAACTCATCAGAC AACAAGCTTTTATTCAACATTACGATCA	312	1–312
		U19	GTTTTCCCAGTCACGTTGTA
	Ct	F R	^§CGA GACTGTAAAGAACTGAATCCTG AATAAGCTTTCAACAGGTACCACAGTCA	120	1131–1251
		T7	TTGTGAGGGGATAACAATTTC
	S	F R	GTGACGCGGTGCAGGGCG ATTTAGGTGACACTATAG
pGEX-6P-1	Nt	F R	^£ACCCGAATTC CCAATGAACAAACTGATAAGAC ^#AACGCGGCCGC TTATTCAACATTACGATCA	312	1–312
	Ct	F R	^¥AGACGGATCC TGGACTGTGAAAGAACTG ^#AATGCGGCCGC TCAACAGGTACCACAGTCA	120	1131–1251
	S	F R	CCAGCAAGTATATAGCATGG CCGGGAGCTGCATGTGTCAGAGG

^a: F, forward, R, reverse, S, sequencing primer. ^b: Listed 5'-3'. The sequences in bold are primers restriction enzymes used for cloning (^§ :Nru I, ^£: Eco RI,^#: Not I, ^¥:BamH I). ^c: Locations of DNA fragments are according to Genbank accession number [EMBL: X55903].

ISSN: 1471-2180