Figure 2

Blocking of binding of mAbFim2 and mAbFim3 to Fim2-expressing strain S1 (left) and of mAbFim3 and mAbFim2 to Fim3-expressing strain S3 (right) by human IgG antibodies to fimbriae. Patients 48I and 10II were infected by Fim2 strains and patient 2026 by Fim3 strain. Blocking of binding of mAbFim2 and mAbFim3 to S1 and S3 by normal sheep serum in PBS (NSS-PBS) was not observed. Data were from a single experiment. Three sera with different blocking levels were tested twice and the results obtained were same. In each experiment, both positive and negative controls were included, and all the experiments were performed by an experienced technician.